1fcw

[[Image:1fcw.gif|left|200px]]

|PDB= 1fcw |SIZE=350|CAPTION= <scene name='initialview01'>1fcw</scene>, resolution 17.0&Aring;

|SITE=

|LIGAND= <scene name='pdbligand=1MA:6-HYDRO-1-METHYLADENOSINE-5&#39;-MONOPHOSPHATE'>1MA</scene>, <scene name='pdbligand=2MG:2N-METHYLGUANOSINE-5&#39;-MONOPHOSPHATE'>2MG</scene>, <scene name='pdbligand=5MC:5-METHYLCYTIDINE-5&#39;-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5&#39;-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5&#39;-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5&#39;-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=M2G:N2-DIMETHYLGUANOSINE-5&#39;-MONOPHOSPHATE'>M2G</scene>, <scene name='pdbligand=OMC:O2&#39;-METHYLYCYTIDINE-5&#39;-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2&#39;-METHYLGUANOSINE-5&#39;-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5&#39;-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=YG:WYBUTOSINE'>YG</scene>

|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fcw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fcw OCA], [http://www.ebi.ac.uk/pdbsum/1fcw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fcw RCSB]</span>

 

 

==Overview==

Three-dimensional cryomaps have been reconstructed for tRNA-ribosome complexes in pre- and posttranslocational states at 17-A resolution. The positions of tRNAs in the A and P sites in the pretranslocational complexes and in the P and E sites in the posttranslocational complexes have been determined. Of these, the P-site tRNA position is the same as seen earlier in the initiation-like fMet-tRNA(f)(Met)-ribosome complex, where it was visualized with high accuracy. Now, the positions of the A- and E-site tRNAs are determined with similar accuracy. The positions of the CCA end of the tRNAs at the A site are different before and after peptide bond formation. The relative positions of anticodons of P- and E-site tRNAs in the posttranslocational state are such that a codon-anticodon interaction at the E site appears feasible.

 

1FCW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FCW OCA].

 

Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle., Agrawal RK, Spahn CM, Penczek P, Grassucci RA, Nierhaus KH, Frank J, J Cell Biol. 2000 Aug 7;150(3):447-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10931859 10931859]

[[Category: Protein complex]]

[[Category: Agrawal, R K.]]

[[Category: Frank, J.]]

[[Category: Grassucci, R A.]]

[[Category: Nierhaus, K H.]]

[[Category: Penczek, P.]]

[[Category: Spahn, C M.T.]]