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1r1u

From Proteopedia

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Current revision

Crystal structure of the metal-sensing transcriptional repressor CzrA from Staphylococcus aureus in the apo-form

Structural highlights

1r1u is a 4 chain structure with sequence from Staphylococcus aureus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O85142_STAAU

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The origin of metal ion selectivity by members of the SmtB/ArsR family of bacterial metal-sensing transcriptional repressors and the mechanism of negative allosteric regulation of DNA binding is poorly understood. Here, we report that two homologous zinc sensors, Staphylococcus aureus CzrA and cyanobacterial SmtB, are "winged" helix homodimeric DNA-binding proteins that bind Zn(II) to a pair of tetrahedral, interhelical binding sites, with two ligands derived from the alpha5 helix of one subunit, Asp84 O(delta1) (Asp104 in SmtB), His86 N(delta1) (His106), and two derived from the alpha5 helix of the other, His97' N(delta1) (His117') and His100' N(epsilon2) (Glu120'). Formation of the metal chelate drives a quaternary structural switch mediated by an intersubunit hydrogen-binding network that originates with the non-liganding N(epsilon2) face of His97 in CzrA (His117 in SmtB) that stabilizes a low-affinity, DNA-binding conformation. The structure of the Zn(1) SmtB homodimer shows that both metal-binding sites of the dimer must be occupied for the quaternary structural switch to occur. Thus, a critical zinc-ligating histidine residue obligatorily couples formation of the metal-sensing coordination chelate to changes in the conformation and dynamics of the putative DNA-binding helices.

A metal-ligand-mediated intersubunit allosteric switch in related SmtB/ArsR zinc sensor proteins.,Eicken C, Pennella MA, Chen X, Koshlap KM, VanZile ML, Sacchettini JC, Giedroc DP J Mol Biol. 2003 Oct 31;333(4):683-95. PMID:14568530^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Eicken C, Pennella MA, Chen X, Koshlap KM, VanZile ML, Sacchettini JC, Giedroc DP. A metal-ligand-mediated intersubunit allosteric switch in related SmtB/ArsR zinc sensor proteins. J Mol Biol. 2003 Oct 31;333(4):683-95. PMID:14568530

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1r1u"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the metal-sensing transcriptional repressor CzrA from Staphylococcus aureus in the apo-form==
-<StructureSection load='1r1u' size='340' side='right' caption='[[1r1u]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='1r1u' size='340' side='right'caption='[[1r1u]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1r1u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R1U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R1U FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1r1u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R1U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R1U FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1smt|1smt]], [[1r1t|1r1t]], [[1r1v|1r1v]], [[1r22|1r22]], [[1r23|1r23]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r1u OCA], [http://pdbe.org/1r1u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r1u RCSB], [http://www.ebi.ac.uk/pdbsum/1r1u PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r1u OCA], [https://pdbe.org/1r1u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r1u RCSB], [https://www.ebi.ac.uk/pdbsum/1r1u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r1u ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/O85142_STAAU O85142_STAAU]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r1/1r1u_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r1/1r1u_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 29: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Chen, X]]
+[[Category: Large Structures]]
-[[Category: Eicken, C]]
+[[Category: Staphylococcus aureus]]
-[[Category: Giedroc, D P]]
+[[Category: Chen X]]
-[[Category: Koshlap, K M]]
+[[Category: Eicken C]]
-[[Category: Pennella, M A]]
+[[Category: Giedroc DP]]
-[[Category: Sacchettini, J C]]
+[[Category: Koshlap KM]]
-[[Category: VanZile, M L]]
+[[Category: Pennella MA]]
-[[Category: Dna binding]]
+[[Category: Sacchettini JC]]
-[[Category: Transcription repressor]]
+[[Category: VanZile ML]]
-[[Category: Transcriptional regulation]]
-[[Category: Winged hth protein]]

1r1u

From Proteopedia

Current revision

Crystal structure of the metal-sensing transcriptional repressor CzrA from Staphylococcus aureus in the apo-form

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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