This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1fdi

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:13, 7 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1fdi.gif|left|200px]]
 
-
{{Structure
+
==OXIDIZED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI COMPLEXED WITH THE INHIBITOR NITRITE==
-
|PDB= 1fdi |SIZE=350|CAPTION= <scene name='initialview01'>1fdi</scene>, resolution 2.9&Aring;
+
<StructureSection load='1fdi' size='340' side='right'caption='[[1fdi]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
-
|SITE= <scene name='pdbsite=FS4:The+Fe+S+Cluster+Is+Coordinated+To+The+S+Atoms+Of+CYS+8,+...'>FS4</scene> and <scene name='pdbsite=MO4:The+Mo+Atom+Is+Coordinated+To+The+Se+Atom+Of+Sec+140,+Th+...'>MO4</scene>
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=6MO:MOLYBDENUM(VI)+ION'>6MO</scene>, <scene name='pdbligand=CSE:SELENOCYSTEINE'>CSE</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=NO2:NITRITE+ION'>NO2</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>
+
<table><tr><td colspan='2'>[[1fdi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FDI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FDI FirstGlance]. <br>
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] </span>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
-
|GENE= FDHF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6MO:MOLYBDENUM(VI)+ION'>6MO</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=NO2:NITRITE+ION'>NO2</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fdi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fdi OCA], [https://pdbe.org/1fdi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fdi RCSB], [https://www.ebi.ac.uk/pdbsum/1fdi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fdi ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fdi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fdi OCA], [http://www.ebi.ac.uk/pdbsum/1fdi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fdi RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/FDHF_ECOLI FDHF_ECOLI] Decomposes formic acid to hydrogen and carbon dioxide under anaerobic conditions in the absence of exogenous electron acceptors.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fd/1fdi_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fdi ConSurf].
 +
<div style="clear:both"></div>
-
'''OXIDIZED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI COMPLEXED WITH THE INHIBITOR NITRITE'''
+
==See Also==
-
 
+
*[[Formate dehydrogenase 3D structures|Formate dehydrogenase 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
Formate dehydrogenase H from Escherichia coli contains selenocysteine (SeCys), molybdenum, two molybdopterin guanine dinucleotide (MGD) cofactors, and an Fe4S4 cluster at the active site and catalyzes the two-electron oxidation of formate to carbon dioxide. The crystal structures of the oxidized [Mo(VI), Fe4S4(ox)] form of formate dehydrogenase H (with and without bound inhibitor) and the reduced [Mo(IV), Fe4S4(red)] form have been determined, revealing a four-domain alphabeta structure with the molybdenum directly coordinated to selenium and both MGD cofactors. These structures suggest a reaction mechanism that directly involves SeCys140 and His141 in proton abstraction and the molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron transfer.
+
-
 
+
-
==About this Structure==
+
-
1FDI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FDI OCA].
+
-
 
+
-
==Reference==
+
-
Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster., Boyington JC, Gladyshev VN, Khangulov SV, Stadtman TC, Sun PD, Science. 1997 Feb 28;275(5304):1305-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9036855 9036855]
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Formate dehydrogenase]]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Boyington JC]]
-
[[Category: Boyington, J C.]]
+
[[Category: Sun PD]]
-
[[Category: Sun, P D.]]
+
-
[[Category: anaerobic]]
+
-
[[Category: dehydrogenase]]
+
-
[[Category: fe4s4]]
+
-
[[Category: formate]]
+
-
[[Category: iron sulfur cluster]]
+
-
[[Category: mgd]]
+
-
[[Category: molybdenum]]
+
-
[[Category: molybdopterin]]
+
-
[[Category: molybdopterin guanine dinucleotide]]
+
-
[[Category: mpt]]
+
-
[[Category: oxidoreductase]]
+
-
[[Category: secy]]
+
-
[[Category: selenium]]
+
-
[[Category: selenocysteine]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:20:50 2008''
+

Current revision

OXIDIZED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI COMPLEXED WITH THE INHIBITOR NITRITE

PDB ID 1fdi

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1fdi"
Personal tools