1ffh

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[[Image:1ffh.gif|left|200px]]
 
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{{Structure
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==N AND GTPASE DOMAINS OF THE SIGNAL SEQUENCE RECOGNITION PROTEIN FFH FROM THERMUS AQUATICUS==
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|PDB= 1ffh |SIZE=350|CAPTION= <scene name='initialview01'>1ffh</scene>, resolution 2.05&Aring;
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<StructureSection load='1ffh' size='340' side='right'caption='[[1ffh]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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|SITE= <scene name='pdbsite=I:Gtpase+Binding+Site+Identified+By+Conserved+Motifs+I-Iv'>I</scene>, <scene name='pdbsite=II:Gtpase+Binding+Site+Identified+By+Conserved+Motifs+I-Iv'>II</scene>, <scene name='pdbsite=III:Gtpase+Binding+Site+Identified+By+Conserved+Motifs+I-Iv'>III</scene> and <scene name='pdbsite=IV:Gtpase+Binding+Site+Identified+By+Conserved+Motifs+I-Iv'>IV</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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<table><tr><td colspan='2'>[[1ffh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FFH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FFH FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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|GENE= FFH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271 Thermus aquaticus])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ffh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ffh OCA], [https://pdbe.org/1ffh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ffh RCSB], [https://www.ebi.ac.uk/pdbsum/1ffh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ffh ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ffh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ffh OCA], [http://www.ebi.ac.uk/pdbsum/1ffh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ffh RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/SRP54_THEAQ SRP54_THEAQ] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY (By similarity).[HAMAP-Rule:MF_00306]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ff/1ffh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ffh ConSurf].
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<div style="clear:both"></div>
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'''N AND GTPASE DOMAINS OF THE SIGNAL SEQUENCE RECOGNITION PROTEIN FFH FROM THERMUS AQUATICUS'''
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==See Also==
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*[[Signal recognition particle 3D structures|Signal recognition particle 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The signal-recognition particle (SRP) and its receptor (SR) function in the co-translational targeting of nascent protein-ribosome complexes to the membrane translocation apparatus. The SRP protein subunit (termed Ffh in bacteria) that recognizes the signal sequence of nascent polypeptides is a GTPase, as is the SR-alpha subunit (termed FtsY). Ffh and FtsY interact directly, each stimulating the GTP hydrolysis activity of the other. The sequence of Ffh suggests three domains: an amino-terminal N domain of unknown function, a central GTPase G domain, and a methionine-rich M domain that binds both SRP RNA and signal peptides. Sequence conservation suggests that structurally similar N and G domains are present in FtsY. Here we report the structure of the nucleotide-free form of the NG fragment of Ffh. Consistent with a role for apo Ffh in protein targeting, the side chains of the empty active-site pocket form a tight network of interactions which may stabilize the nucleotide-free protein. The structural relationship between the two domains suggests that the N domain senses or controls the nucleotide occupancy of the GTPase domain. A structural subdomain unique to these evolutionarily conserved GTPases constitutes them as a distinct subfamily in the GTPase superfamily.
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[[Category: Large Structures]]
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==About this Structure==
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1FFH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FFH OCA].
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==Reference==
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Structure of the conserved GTPase domain of the signal recognition particle., Freymann DM, Keenan RJ, Stroud RM, Walter P, Nature. 1997 Jan 23;385(6614):361-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9002524 9002524]
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[[Category: Single protein]]
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[[Category: Thermus aquaticus]]
[[Category: Thermus aquaticus]]
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[[Category: Freymann, D M.]]
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[[Category: Freymann DM]]
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[[Category: Keenan, R J.]]
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[[Category: Keenan RJ]]
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[[Category: Stroud, R M.]]
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[[Category: Stroud RM]]
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[[Category: Walter, P.]]
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[[Category: Walter P]]
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[[Category: ffh]]
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[[Category: gtpase]]
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[[Category: ribonucleoprotein]]
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[[Category: signal recognition particle]]
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[[Category: srp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:21:48 2008''
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Current revision

N AND GTPASE DOMAINS OF THE SIGNAL SEQUENCE RECOGNITION PROTEIN FFH FROM THERMUS AQUATICUS

PDB ID 1ffh

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