This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1fhl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:14, 20 March 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1fhl.gif|left|200px]]
 
-
{{Structure
+
==CRYSTAL STRUCTURE OF BETA-1,4-GALACTANASE FROM ASPERGILLUS ACULEATUS AT 293K==
-
|PDB= 1fhl |SIZE=350|CAPTION= <scene name='initialview01'>1fhl</scene>, resolution 2.3&Aring;
+
<StructureSection load='1fhl' size='340' side='right'caption='[[1fhl]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[1fhl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_aculeatus Aspergillus aculeatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FHL FirstGlance]. <br>
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinogalactan_endo-1,4-beta-galactosidase Arabinogalactan endo-1,4-beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.89 3.2.1.89] </span>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-
|GENE=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fhl OCA], [https://pdbe.org/1fhl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fhl RCSB], [https://www.ebi.ac.uk/pdbsum/1fhl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fhl ProSAT]</span></td></tr>
-
|DOMAIN=
+
</table>
-
|RELATEDENTRY=[[1fob|1FOB]]
+
== Function ==
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fhl OCA], [http://www.ebi.ac.uk/pdbsum/1fhl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fhl RCSB]</span>
+
[https://www.uniprot.org/uniprot/GANA_ASPAC GANA_ASPAC]
-
}}
+
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fh/1fhl_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fhl ConSurf].
 +
<div style="clear:both"></div>
-
'''CRYSTAL STRUCTURE OF BETA-1,4-GALACTANASE FROM ASPERGILLUS ACULEATUS AT 293K'''
+
==See Also==
-
 
+
*[[Beta-1%2C4-galactanase|Beta-1%2C4-galactanase]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
The three-dimensional structure of Aspergillus aculeatus beta-1,4-galactanase (AAGAL), an enzyme involved in pectin degradation, has been determined by multiple isomorphous replacement to 2.3 and 1.8 A resolution at 293 and 100 K, respectively. It represents the first known structure for a polysaccharidase with this specificity and for a member of glycoside hydrolase family 53 (GH-53). The enzyme folds into a (beta/alpha)(8) barrel with the active site cleft located at the C-terminal side of the barrel consistent with the classification of GH-53 in clan GH-A, a superfamily of enzymes with common fold and catalytic machinery but diverse specificities. Putative substrate-enzyme interactions were elucidated by modeling of beta-1,4-linked galactobioses into the possible substrate binding subsites. The structure and modeling studies identified five potential subsites for the binding of galactans, of which one is a pocket suited for accommodating the arabinan side chain in arabinogalactan, one of the natural substrates. A comparison with the substrate binding grooves of other Clan GH-A enzymes suggests that shape complementarity is crucial in determining the specificity of polysaccharidases.
+
-
 
+
-
==About this Structure==
+
-
1FHL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_aculeatus Aspergillus aculeatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHL OCA].
+
-
 
+
-
==Reference==
+
-
Aspergillus aculeatus beta-1,4-galactanase: substrate recognition and relations to other glycoside hydrolases in clan GH-A., Ryttersgaard C, Lo Leggio L, Coutinho PM, Henrissat B, Larsen S, Biochemistry. 2002 Dec 24;41(51):15135-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12484750 12484750]
+
-
[[Category: Arabinogalactan endo-1,4-beta-galactosidase]]
+
[[Category: Aspergillus aculeatus]]
[[Category: Aspergillus aculeatus]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Larsen, S.]]
+
[[Category: Larsen S]]
-
[[Category: Ryttersgaard, C.]]
+
[[Category: Ryttersgaard C]]
-
[[Category: b/a barrel]]
+
-
[[Category: clan gh-a]]
+
-
[[Category: family 53]]
+
-
[[Category: glycosyl hydrolase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:23:02 2008''
+

Current revision

CRYSTAL STRUCTURE OF BETA-1,4-GALACTANASE FROM ASPERGILLUS ACULEATUS AT 293K

PDB ID 1fhl

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1fhl"
Personal tools