1mkm

<StructureSection load='1mkm' size='340' side='right' caption='[[1mkm]], [[Resolution|resolution]] 2.20&Aring;' scene=''>

<table><tr><td colspan='2'>[[1mkm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1jmr 1jmr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MKM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MKM FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mkm OCA], [http://pdbe.org/1mkm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mkm RCSB], [http://www.ebi.ac.uk/pdbsum/1mkm PDBsum], [http://www.topsan.org/Proteins/MCSG/1mkm TOPSAN]</span></td></tr>

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mk/1mkm_consurf.spt"</scriptWhenChecked>

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== Publication Abstract from PubMed ==

Members of the IclR family of transcription regulators modulate signal-dependent expression of genes involved in carbon metabolism in bacteria and archaea. The Thermotoga maritima TM0065 gene codes for a protein (TM-IclR) that is homologous to the IclR family. We have determined the crystal structure of TM-IclR at 2.2 A resolution using MAD phasing and synchrotron radiation. The protein is composed of two domains: the N-terminal DNA-binding domain contains the winged helix-turn-helix motif, and the C-terminal presumed regulatory domain is involved in binding signal molecule. In a proposed signal-binding site, a bound Zn(2+) ion was found. In the crystal, TM-IclR forms a dimer through interactions between DNA-binding domains. In the dimer, the DNA-binding domains are 2-fold related, but the dimer is asymmetric with respect to the orientation of signal-binding domains. Crystal packing analysis showed that TM-IclR dimers form a tetramer through interactions exclusively by signal-binding domains. A model is proposed for binding of IclR-like factors to DNA, and it suggests that signal-dependent transcription regulation is accomplished by affecting an oligomerization state of IclR and therefore its affinity for DNA target.

Crystal structure of Thermotoga maritima 0065, a member of the IclR transcriptional factor family.,Zhang RG, Kim Y, Skarina T, Beasley S, Laskowski R, Arrowsmith C, Edwards A, Joachimiak A, Savchenko A J Biol Chem. 2002 May 24;277(21):19183-90. Epub 2002 Mar 4. PMID:11877432<ref>PMID:11877432</ref>

== References ==

[[Category: Atcc 43589]]

[[Category: Edwards, A]]

[[Category: Joachimiak, A]]

[[Category: Kim, Y]]

[[Category: Structural genomic]]

[[Category: Savchenko, A]]

[[Category: Skarina, T]]

[[Category: Zhang, R G]]

[[Category: Winged helix-turn-helix]]