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1fmb

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[[Image:1fmb.gif|left|200px]]
 
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{{Structure
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==EIAV PROTEASE COMPLEXED WITH THE INHIBITOR HBY-793==
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|PDB= 1fmb |SIZE=350|CAPTION= <scene name='initialview01'>1fmb</scene>, resolution 1.8&Aring;
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<StructureSection load='1fmb' size='340' side='right'caption='[[1fmb]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=HYB:[2-(2-METHYL-PROPANE-2-SULFONYLMETHYL)-3-NAPHTHALEN-1-YL-PROPIONYL-VALINYL]-PHENYLALANINOL'>HYB</scene>
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<table><tr><td colspan='2'>[[1fmb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Equine_infectious_anemia_virus Equine infectious anemia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FMB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FMB FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/HIV-1_retropepsin HIV-1 retropepsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.16 3.4.23.16] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HYB:[2-(2-METHYL-PROPANE-2-SULFONYLMETHYL)-3-NAPHTHALEN-1-YL-PROPIONYL-VALINYL]-PHENYLALANINOL'>HYB</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fmb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fmb OCA], [https://pdbe.org/1fmb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fmb RCSB], [https://www.ebi.ac.uk/pdbsum/1fmb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fmb ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fmb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fmb OCA], [http://www.ebi.ac.uk/pdbsum/1fmb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fmb RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/POL_EIAVC POL_EIAVC] During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fm/1fmb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fmb ConSurf].
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<div style="clear:both"></div>
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'''EIAV PROTEASE COMPLEXED WITH THE INHIBITOR HBY-793'''
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==See Also==
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*[[Virus protease 3D structures|Virus protease 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Equine infectious anemia virus (EIAV), the causative agent of infectious anemia in horses, is a member of the lentiviral family. The virus-encoded proteinase (PR) processes viral polyproteins into functional molecules during replication and it also cleaves viral nucleocapsid protein during infection. The X-ray structure of a complex of the 154G mutant of EIAV PR with the inhibitor HBY-793 was solved at 1.8 A resolution and refined to a crystallographic R-factor of 0.136. The molecule is a dimer in which the monomers are related by a crystallographic twofold axis. Although both the enzyme and the inhibitor are symmetric, the interactions between the central part of the inhibitor and the active site aspartates are asymmetric, and the inhibitor and the two flaps are partially disordered. The overall fold of EIAV PR is very similar to that of other retroviral proteinases. However, a novel feature of the EIAV PR structure is the appearance of the second alpha-helix in the monomer in a position predicted by the structural template for the family of aspartic proteinases. The parts of the EIAV PR with the highest resemblance to human immunodeficiency virus type 1 PR include the substrate-binding sites; thus, the differences in the specificity of both enzymes have to be explained by enzyme-ligand interactions at the periphery of the active site as well.
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==About this Structure==
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1FMB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Equine_infectious_anemia_virus Equine infectious anemia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FMB OCA].
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==Reference==
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Structure of equine infectious anemia virus proteinase complexed with an inhibitor., Gustchina A, Kervinen J, Powell DJ, Zdanov A, Kay J, Wlodawer A, Protein Sci. 1996 Aug;5(8):1453-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8844837 8844837]
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[[Category: Equine infectious anemia virus]]
[[Category: Equine infectious anemia virus]]
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[[Category: HIV-1 retropepsin]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Gustchina A]]
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[[Category: Gustchina, A.]]
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[[Category: Kervinen J]]
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[[Category: Kervinen, J.]]
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[[Category: Wlodawer A]]
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[[Category: Wlodawer, A.]]
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[[Category: Zdanov A]]
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[[Category: Zdanov, A.]]
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[[Category: aspartyl protease]]
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[[Category: endonuclease]]
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[[Category: hydrolase (acid proteinase)]]
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[[Category: polyprotein]]
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[[Category: rna-directed dna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:25:42 2008''
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Current revision

EIAV PROTEASE COMPLEXED WITH THE INHIBITOR HBY-793

PDB ID 1fmb

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