|
|
| (2 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | + | |
| | ==Fungal lectin CGL3 in complex with chitotriose (chitotetraose)== | | ==Fungal lectin CGL3 in complex with chitotriose (chitotetraose)== |
| - | <StructureSection load='2r0h' size='340' side='right' caption='[[2r0h]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='2r0h' size='340' side='right'caption='[[2r0h]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2r0h]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R0H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2R0H FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2r0h]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R0H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R0H FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTO:TRIACETYLCHITOTRIOSE'>CTO</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ulf|1ulf]], [[1ulc|1ulc]], [[1ulg|1ulg]], [[1uld|1uld]], [[1ule|1ule]], [[1ul9|1ul9]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900017:triacetyl-beta-chitotriose'>PRD_900017</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cgl3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5346 Coprinopsis cinerea])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r0h OCA], [https://pdbe.org/2r0h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r0h RCSB], [https://www.ebi.ac.uk/pdbsum/2r0h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r0h ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r0h OCA], [http://pdbe.org/2r0h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2r0h RCSB], [http://www.ebi.ac.uk/pdbsum/2r0h PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CGL3_COPCI CGL3_COPCI]] Binds complex carbohydrates, such as chitooligosaccharides. Does not bind lactose. May play a role in fruiting body formation.<ref>PMID:18440554</ref> | + | [https://www.uniprot.org/uniprot/CGL3_COPCI CGL3_COPCI] Binds complex carbohydrates, such as chitooligosaccharides. Does not bind lactose. May play a role in fruiting body formation.<ref>PMID:18440554</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r0/2r0h_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r0/2r0h_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| Line 34: |
Line 34: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Coprinopsis cinerea]] | | [[Category: Coprinopsis cinerea]] |
| - | [[Category: Aebi, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Ban, N]] | + | [[Category: Aebi M]] |
| - | [[Category: Gruenler, A]] | + | [[Category: Ban N]] |
| - | [[Category: Kuenzler, M]] | + | [[Category: Gruenler A]] |
| - | [[Category: Thore, S]] | + | [[Category: Kuenzler M]] |
| - | [[Category: Waelti, M A]] | + | [[Category: Thore S]] |
| - | [[Category: Walser, P J]] | + | [[Category: Waelti MA]] |
| - | [[Category: Chitooligosaccharide]]
| + | [[Category: Walser PJ]] |
| - | [[Category: Chitotriose]]
| + | |
| - | [[Category: Galectin-related]]
| + | |
| - | [[Category: Lectin]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
Function
CGL3_COPCI Binds complex carbohydrates, such as chitooligosaccharides. Does not bind lactose. May play a role in fruiting body formation.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Recent advances in genome sequencing efforts have revealed an abundance of novel putative lectins. Among these, many galectin-related proteins, characterized by many conserved residues but intriguingly lacking critical amino acids, have been found in all corners of the eukaryotic superkingdom. Here we present a structural and biochemical analysis of one representative, the galectin-related lectin CGL3 found in the inky cap mushroom Coprinopsis cinerea. This protein contains all but one conserved residues known to be involved in beta-galactoside binding in galectins. A Trp residue strictly conserved among galectins is changed to an Arg in CGL3 (R81). Accordingly, the galectin-related protein is not able to bind lactose. Screening of a glycan array revealed that CGL3 displays preference for oligomers of beta1-4-linked N-acetyl-glucosamines (chitooligosaccharides) and GalNAc beta 1-4GlcNAc (LacdiNAc). Carbohydrate-binding affinity of this novel lectin was quantified using isothermal titration calorimetry, and its mode of chitooligosaccharide coordination not involving any aromatic amino acid residues was studied by X-ray crystallography. Structural information was used to alter the carbohydrate-binding specificity and substrate affinity of CGL3. The importance of residue R81 in determining the carbohydrate-binding specificity was demonstrated by replacing this Arg with a Trp residue (R81W). This single-amino-acid change led to a lectin that failed to bind chitooligosaccharides but gained lactose binding. Our results demonstrate that, similar to the legume lectin fold, the galectin fold represents a conserved structural framework upon which dramatically altered specificities can be grafted by few alterations in the binding site and that, in consequence, many metazoan galectin-related proteins may represent lectins with novel carbohydrate-binding specificities.
Structural basis for chitotetraose coordination by CGL3, a novel galectin-related protein from Coprinopsis cinerea.,Walti MA, Walser PJ, Thore S, Grunler A, Bednar M, Kunzler M, Aebi M J Mol Biol. 2008 May 23;379(1):146-59. Epub 2008 Apr 3. PMID:18440554[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Walti MA, Walser PJ, Thore S, Grunler A, Bednar M, Kunzler M, Aebi M. Structural basis for chitotetraose coordination by CGL3, a novel galectin-related protein from Coprinopsis cinerea. J Mol Biol. 2008 May 23;379(1):146-59. Epub 2008 Apr 3. PMID:18440554 doi:10.1016/j.jmb.2008.03.062
- ↑ Walti MA, Walser PJ, Thore S, Grunler A, Bednar M, Kunzler M, Aebi M. Structural basis for chitotetraose coordination by CGL3, a novel galectin-related protein from Coprinopsis cinerea. J Mol Biol. 2008 May 23;379(1):146-59. Epub 2008 Apr 3. PMID:18440554 doi:10.1016/j.jmb.2008.03.062
|
