5b2o

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(New page: '''Unreleased structure''' The entry 5b2o is ON HOLD Authors: Hirano, H., Nishimasu, H., Nakane, T, Ishitani, R., Nureki, O. Description: Crystal structure of Francisella novicida Cas9...)
Current revision (09:07, 20 March 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 5b2o is ON HOLD
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==Crystal structure of Francisella novicida Cas9 in complex with sgRNA and target DNA (TGG PAM)==
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<StructureSection load='5b2o' size='340' side='right'caption='[[5b2o]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5b2o]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida Francisella tularensis subsp. novicida], [https://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida_U112 Francisella tularensis subsp. novicida U112] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B2O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B2O FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b2o OCA], [https://pdbe.org/5b2o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b2o RCSB], [https://www.ebi.ac.uk/pdbsum/5b2o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b2o ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAS9_FRATN CAS9_FRATN] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity (By similarity). Cuts target DNA when Cas9 and gRNAs are mixed.<ref>PMID:24270795</ref>
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Authors: Hirano, H., Nishimasu, H., Nakane, T, Ishitani, R., Nureki, O.
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==See Also==
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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Description: Crystal structure of Francisella novicida Cas9 in complex with sgRNA and target DNA (TGG PAM)
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== References ==
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[[Category: Unreleased Structures]]
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<references/>
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[[Category: Nureki, O]]
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__TOC__
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[[Category: Nishimasu, H]]
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</StructureSection>
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[[Category: Hirano, H]]
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[[Category: Francisella tularensis subsp. novicida]]
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[[Category: Nakane, T, Ishitani, R]]
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[[Category: Francisella tularensis subsp. novicida U112]]
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[[Category: Large Structures]]
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[[Category: Synthetic construct]]
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[[Category: Hirano H]]
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[[Category: Ishitani R]]
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[[Category: Nakane T]]
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[[Category: Nishimasu H]]
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[[Category: Nureki O]]

Current revision

Crystal structure of Francisella novicida Cas9 in complex with sgRNA and target DNA (TGG PAM)

PDB ID 5b2o

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