5huq

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==A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase==
==A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase==
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<StructureSection load='5huq' size='340' side='right' caption='[[5huq]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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<StructureSection load='5huq' size='340' side='right'caption='[[5huq]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5huq]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4yns 4yns]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HUQ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5huq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactiplantibacillus_plantarum Lactiplantibacillus plantarum]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4yns 4yns]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HUQ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4EY:1-[(2R,3R,4S,5R)-3,4-BIS(OXIDANYL)-5-(PHOSPHONOOXYMETHYL)OXOLAN-2-YL]-5-METHANETHIOYL-PYRIDINE-3-CARBOTHIOIC+S-ACID'>4EY</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5huq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5huq OCA], [http://pdbe.org/5huq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5huq RCSB], [http://www.ebi.ac.uk/pdbsum/5huq PDBsum]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4EY:1-[(2R,3R,4S,5R)-3,4-BIS(OXIDANYL)-5-(PHOSPHONOOXYMETHYL)OXOLAN-2-YL]-5-METHANETHIOYL-PYRIDINE-3-CARBOTHIOIC+S-ACID'>4EY</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5huq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5huq OCA], [https://pdbe.org/5huq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5huq RCSB], [https://www.ebi.ac.uk/pdbsum/5huq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5huq ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/LARA_LACPL LARA_LACPL] Catalyzes the interconversion between the D- and L-isomers of lactate (PubMed:24710389, PubMed:26138974). May act as a rescue enzyme to ensure D-lactate production in physiological conditions where its production by the D-lactate dehydrogenase LdhD is not sufficient (PubMed:16166538). D-Lactate is absolutely required for growth of L.plantarum and is an essential component of the cell wall peptidoglycan in this species, where it is incorporated as the last residue of the muramoyl-pentadepsipeptide peptidoglycan precursor; its incorporation confers high level of vancomycin resistance (PubMed:16166538).<ref>PMID:16166538</ref> <ref>PMID:24710389</ref> <ref>PMID:26138974</ref>
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Lactic acid racemization is involved in lactate metabolism and cell wall assembly of many microorganisms. Lactate racemase (Lar) requires nickel, but the nickel-binding site and the role of three accessory proteins required for its activation remain enigmatic. We combined mass spectrometry and x-ray crystallography to show that Lar from Lactobacillus plantarum possesses an organometallic nickel-containing prosthetic group. A nicotinic acid mononucleotide derivative is tethered to Lys(184) and forms a tridentate pincer complex that coordinates nickel through one metal-carbon and two metal-sulfur bonds, with His(200) as another ligand. Although similar complexes have been previously synthesized, there was no prior evidence for the existence of pincer cofactors in enzymes. The wide distribution of the accessory proteins without Lar suggests that it may play a role in other enzymes.
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METALLOPROTEINS. A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase.,Desguin B, Zhang T, Soumillion P, Hols P, Hu J, Hausinger RP Science. 2015 Jul 3;349(6243):66-9. doi: 10.1126/science.aab2272. PMID:26138974<ref>PMID:26138974</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5huq" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Desguin, B]]
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[[Category: Lactiplantibacillus plantarum]]
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[[Category: Hausinger, R P]]
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[[Category: Large Structures]]
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[[Category: Hols, P]]
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[[Category: Desguin B]]
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[[Category: Hu, J]]
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[[Category: Hausinger RP]]
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[[Category: Soumillion, P]]
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[[Category: Hols P]]
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[[Category: Zhang, T]]
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[[Category: Hu J]]
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[[Category: Cofactor]]
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[[Category: Soumillion P]]
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[[Category: Isomerase]]
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[[Category: Zhang T]]
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[[Category: Nickel]]
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[[Category: Racemase]]
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Current revision

A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase

PDB ID 5huq

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