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1g5a

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AMYLOSUCRASE FROM NEISSERIA POLYSACCHAREA

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Retrieved from "http://52.214.119.220/wiki/index.php/1g5a"

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-[[Image:1g5a.jpg|left|200px]]
- {{Structure
+==AMYLOSUCRASE FROM NEISSERIA POLYSACCHAREA==
-|PDB= 1g5a |SIZE=350|CAPTION= <scene name='initialview01'>1g5a</scene>, resolution 1.40&Aring;
+<StructureSection load='1g5a' size='340' side='right'caption='[[1g5a]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
+<table><tr><td colspan='2'>[[1g5a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_polysaccharea Neisseria polysaccharea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G5A FirstGlance]. <br>
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Amylosucrase Amylosucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.4 2.4.1.4] </span>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5a OCA], [https://pdbe.org/1g5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g5a RCSB], [https://www.ebi.ac.uk/pdbsum/1g5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g5a ProSAT]</span></td></tr>
-|RELATEDENTRY=
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5a OCA], [http://www.ebi.ac.uk/pdbsum/1g5a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1g5a RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/AMYS_NEIPO AMYS_NEIPO] Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.<ref>PMID:9882648</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/1g5a_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g5a ConSurf].
+<div style="clear:both"></div>
-'''AMYLOSUCRASE FROM NEISSERIA POLYSACCHAREA'''
+==See Also==
+*[[Amylase 3D structures|Amylase 3D structures]]
+== References ==
-==Overview==
+<references/>
-Amylosucrase (E.C. 2.4.1.4) is a member of Family 13 of the glycoside hydrolases (the alpha-amylases), although its biological function is the synthesis of amylose-like polymers from sucrose. The structure of amylosucrase from Neisseria polysaccharea is divided into five domains: an all helical N-terminal domain that is not similar to any known fold, a (beta/alpha)(8)-barrel A-domain, B- and B'-domains displaying alpha/beta-structure, and a C-terminal eight-stranded beta-sheet domain. In contrast to other Family 13 hydrolases that have the active site in the bottom of a large cleft, the active site of amylosucrase is at the bottom of a pocket at the molecular surface. A substrate binding site resembling the amylase 2 subsite is not found in amylosucrase. The site is blocked by a salt bridge between residues in the second and eight loops of the (beta/alpha)(8)-barrel. The result is an exo-acting enzyme. Loop 7 in the amylosucrase barrel is prolonged compared with the loop structure found in other hydrolases, and this insertion (forming domain B') is suggested to be important for the polymer synthase activity of the enzyme. The topology of the B'-domain creates an active site entrance with several ravines in the molecular surface that could be used specifically by the substrates/products (sucrose, glucan polymer, and fructose) that have to get in and out of the active site pocket.
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-G5A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Neisseria_polysaccharea Neisseria polysaccharea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5A OCA].
-==Reference==
-Amylosucrase, a glucan-synthesizing enzyme from the alpha-amylase family., Skov LK, Mirza O, Henriksen A, De Montalk GP, Remaud-Simeon M, Sarcabal P, Willemot RM, Monsan P, Gajhede M, J Biol Chem. 2001 Jul 6;276(27):25273-8. Epub 2001 Apr 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11306569 11306569]
-[[Category: Amylosucrase]]
 [[Category: Neisseria polysaccharea]]
-[[Category: Single protein]]
+[[Category: De Montalk GP]]
-[[Category: Gajhede, M.]]
+[[Category: Gajhede M]]
-[[Category: Henriksen, A.]]
+[[Category: Henriksen A]]
-[[Category: Mirza, O.]]
+[[Category: Mirza O]]
-[[Category: Monsan, P.]]
+[[Category: Monsan P]]
-[[Category: Montalk, G P.De.]]
+[[Category: Remaud-Simeon M]]
-[[Category: Remaud-Simeon, M.]]
+[[Category: Sarcabal P]]
-[[Category: Sarcabal, P.]]
+[[Category: Skov LK]]
-[[Category: Skov, L K.]]
+[[Category: Willemot R-M]]
-[[Category: Willemot, R M.]]
-[[Category: (beta-alpha)8 barrel]]
-[[Category: glycoside hydrolase]]
-[[Category: glycosyltransferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:36:54 2008''

1g5a

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AMYLOSUCRASE FROM NEISSERIA POLYSACCHAREA

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Function

Evolutionary Conservation

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