1g5q

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[[Image:1g5q.jpg|left|200px]]
 
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{{Structure
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==EPID H67N COMPLEXED WITH SUBSTRATE PEPTIDE DSYTC==
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|PDB= 1g5q |SIZE=350|CAPTION= <scene name='initialview01'>1g5q</scene>, resolution 2.57&Aring;
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<StructureSection load='1g5q' size='340' side='right'caption='[[1g5q]], [[Resolution|resolution]] 2.57&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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<table><tr><td colspan='2'>[[1g5q]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G5Q FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.57&#8491;</td></tr>
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|GENE= EPID ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1282 Staphylococcus epidermidis])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5q OCA], [https://pdbe.org/1g5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g5q RCSB], [https://www.ebi.ac.uk/pdbsum/1g5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g5q ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1g63|1G63]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5q OCA], [http://www.ebi.ac.uk/pdbsum/1g5q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1g5q RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/EPID_STAEP EPID_STAEP] Catalyzes the removal of two reducing equivalents (oxidative decarboxylation) from the cysteine residue of the C-terminal meso-lanthionine of epidermin to form a --C==C-- double bond.
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== Evolutionary Conservation ==
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'''EPID H67N COMPLEXED WITH SUBSTRATE PEPTIDE DSYTC'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/1g5q_consurf.spt"</scriptWhenChecked>
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Epidermin from Staphylococcus epidermidis Tu3298 is an antimicrobial peptide of the lantibiotic family that contains, amongst other unusual amino acids, S:-[(Z:)- 2-aminovinyl]-D-cysteine. This residue is introduced by post-translational modification of the ribosomally synthesized precursor EpiA. Modification starts with the oxidative decarboxylation of its C-terminal cysteine by the flavoprotein EpiD generating a reactive (Z:)-enethiol intermediate. We have determined the crystal structures of EpiD and EpiD H67N in complex with the substrate pentapeptide DSYTC at 2.5 A resolution. Rossmann-type monomers build up a dodecamer of 23 point symmetry with trimers disposed at the vertices of a tetrahedron. Oligomer formation is essential for binding of flavin mononucleotide and substrate, which is buried by an otherwise disordered substrate recognition clamp. A pocket for the tyrosine residue of the substrate peptide is formed by an induced fit mechanism. The substrate contacts flavin mononucleotide only via Cys-Sgamma, suggesting its oxidation as the initial step. A thioaldehyde intermediate could undergo spontaneous decarboxylation. The unusual substrate recognition mode and the type of chemical reaction performed provide insight into a novel family of flavoproteins.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1G5Q is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5Q OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g5q ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Crystal structure of the peptidyl-cysteine decarboxylase EpiD complexed with a pentapeptide substrate., Blaesse M, Kupke T, Huber R, Steinbacher S, EMBO J. 2000 Dec 1;19(23):6299-310. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11101502 11101502]
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</StructureSection>
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[[Category: Protein complex]]
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[[Category: Large Structures]]
[[Category: Staphylococcus epidermidis]]
[[Category: Staphylococcus epidermidis]]
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[[Category: Blaesse, M.]]
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[[Category: Blaesse M]]
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[[Category: Huber, R.]]
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[[Category: Huber R]]
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[[Category: Kupke, T.]]
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[[Category: Kupke T]]
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[[Category: Steinbacher, S.]]
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[[Category: Steinbacher S]]
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[[Category: alpha]]
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[[Category: beta protein]]
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[[Category: rossman like fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:37:08 2008''
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Current revision

EPID H67N COMPLEXED WITH SUBSTRATE PEPTIDE DSYTC

PDB ID 1g5q

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