1g5n

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[[Image:1g5n.jpg|left|200px]]
 
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{{Structure
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==ANNEXIN V COMPLEX WITH HEPARIN OLIGOSACCHARIDES==
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|PDB= 1g5n |SIZE=350|CAPTION= <scene name='initialview01'>1g5n</scene>, resolution 1.9&Aring;
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<StructureSection load='1g5n' size='340' side='right'caption='[[1g5n]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IDU:1,4-DIDEOXY-O2-SULFO-GLUCURONIC+ACID'>IDU</scene>, <scene name='pdbligand=SGN:N,O6-DISULFO-GLUCOSAMINE'>SGN</scene>, <scene name='pdbligand=UAP:1,4-DIDEOXY-5-DEHYDRO-O2-SULFO-GLUCURONIC+ACID'>UAP</scene>
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<table><tr><td colspan='2'>[[1g5n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G5N FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE= LC5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IDS:2-O-SULFO-ALPHA-L-IDOPYRANURONIC+ACID'>IDS</scene>, <scene name='pdbligand=SGN:N,O6-DISULFO-GLUCOSAMINE'>SGN</scene>, <scene name='pdbligand=UAP:4-DEOXY-2-O-SULFO-ALPHA-L-THREO-HEX-4-ENOPYRANURONIC+ACID'>UAP</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5n OCA], [https://pdbe.org/1g5n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g5n RCSB], [https://www.ebi.ac.uk/pdbsum/1g5n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g5n ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5n OCA], [http://www.ebi.ac.uk/pdbsum/1g5n PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1g5n RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/ANXA5_RAT ANXA5_RAT] This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/1g5n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g5n ConSurf].
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<div style="clear:both"></div>
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'''ANNEXIN V COMPLEX WITH HEPARIN OLIGOSACCHARIDES'''
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==See Also==
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*[[Annexin 3D structures|Annexin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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BACKGROUND: Annexin V, an abundant anticoagulant protein, has been proposed to exert its effects by self-assembling into highly ordered arrays on phospholipid membranes to form a protective anti-thrombotic shield at the cell surface. The protein exhibits very high-affinity calcium-dependent interactions with acidic phospholipid membranes, as well as specific binding to glycosaminoglycans (GAGs) such as heparin and heparan sulfate, a major component of cell surface proteoglycans. At present, there is no structural information to elucidate this interaction or the role it may play in annexin V function at the cell surface. RESULTS: We report the 1.9 A crystal structure of annexin V in complex with heparin-derived tetrasaccharides. This structure represents the first of a heparin oligosaccharide binding to a protein where calcium ions are essential for the interaction. Two distinct GAG binding sites are situated on opposite protein surfaces. Basic residues at each site were identified from the structure and site-directed mutants were prepared. The heparin binding properties of these mutants were measured by surface plasmon resonance. The results confirm the roles of these mutated residues in heparin binding, and the kinetic and thermodynamic data define the functionally distinct character of each distal binding surface. CONCLUSION: The annexin V molecule, as it self-assembles into an organized array on the membrane surface, can bind the heparan sulfate components of cell surface proteoglycans. A novel model is presented in which proteoglycan heparan sulfate could assist in the localization of annexin V to the cell surface membrane and/or stabilization of the entire molecular assembly to promote anticoagulation.
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[[Category: Large Structures]]
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==About this Structure==
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1G5N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5N OCA].
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==Reference==
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Annexin V--heparin oligosaccharide complex suggests heparan sulfate--mediated assembly on cell surfaces., Capila I, Hernaiz MJ, Mo YD, Mealy TR, Campos B, Dedman JR, Linhardt RJ, Seaton BA, Structure. 2001 Jan 10;9(1):57-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11342135 11342135]
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[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Campos B]]
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[[Category: Campos, B.]]
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[[Category: Capila I]]
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[[Category: Capila, I.]]
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[[Category: Dedman JR]]
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[[Category: Dedman, J R.]]
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[[Category: Heraiz MJ]]
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[[Category: Heraiz, M J.]]
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[[Category: Linhardt RJ]]
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[[Category: Linhardt, R J.]]
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[[Category: Mealy TR]]
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[[Category: Mealy, T R.]]
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[[Category: Mo YD]]
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[[Category: Mo, Y D.]]
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[[Category: Seaton BA]]
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[[Category: Seaton, B A.]]
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[[Category: calcium]]
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[[Category: helix-bundle]]
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[[Category: heparan sulfate]]
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[[Category: heparin]]
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[[Category: membrane binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:37:08 2008''
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Current revision

ANNEXIN V COMPLEX WITH HEPARIN OLIGOSACCHARIDES

PDB ID 1g5n

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