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| | ==Mis16 with H4 peptide== | | ==Mis16 with H4 peptide== |
| - | <StructureSection load='4xyi' size='340' side='right' caption='[[4xyi]], [[Resolution|resolution]] 3.00Å' scene=''> | + | <StructureSection load='4xyi' size='340' side='right'caption='[[4xyi]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4xyi]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XYI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4xyi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_japonicus_yFS275 Schizosaccharomyces japonicus yFS275]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XYI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XYI FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xyh|4xyh]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xyi OCA], [http://pdbe.org/4xyi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xyi RCSB], [http://www.ebi.ac.uk/pdbsum/4xyi PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xyi OCA], [https://pdbe.org/4xyi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xyi RCSB], [https://www.ebi.ac.uk/pdbsum/4xyi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xyi ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/B6JV96_SCHJY B6JV96_SCHJY]] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling (By similarity).[SAAS:SAAS00349935] | + | [https://www.uniprot.org/uniprot/B6JV96_SCHJY B6JV96_SCHJY] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling (By similarity).[SAAS:SAAS00349935] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: An, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Cho, U S]] | + | [[Category: Schizosaccharomyces japonicus yFS275]] |
| - | [[Category: Kim, H]] | + | [[Category: An S]] |
| - | [[Category: Cenp-a]] | + | [[Category: Cho U-S]] |
| - | [[Category: Centromere]] | + | [[Category: Kim H]] |
| - | [[Category: Chaperone]]
| + | |
| - | [[Category: Histone]]
| + | |
| - | [[Category: Kinetochore]]
| + | |
| - | [[Category: Mis18 complex]]
| + | |
| Structural highlights
Function
B6JV96_SCHJY Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling (By similarity).[SAAS:SAAS00349935]
Publication Abstract from PubMed
CENP-A is a centromere-specific histone H3 variant that is required for kinetochore assembly and accurate chromosome segregation. For it to function properly, CENP-A must be specifically localized to centromeres. In fission yeast, Scm3sp and the Mis18 complex, composed of Mis16, Eic1, and Mis18, function as a CENP-A(Cnp1)-specific chaperone and a recruiting factor, respectively, and together ensure accurate delivery of CENP-A(Cnp1) to centromeres. Although how Scm3sp specifically recognizes CENP-A(Cnp1) has been revealed recently, the recruiting mechanism of CENP-A(Cnp1) via the Mis18 complex remains unknown. In this study, we have determined crystal structures of Schizosaccharomyces japonicus Mis16 alone and in complex with the helix 1 of histone H4 (H4alpha1). Crystal structures followed by mutant analysis and affinity pull-downs have revealed that Mis16 recognizes both H4alpha1 and Scm3sp independently within the CENP-A(Cnp1)/H4:Scm3sp complex. This observation suggests that Mis16 gains CENP-A(Cnp1) specificity by recognizing both Scm3sp and histone H4. Our studies provide insights into the molecular mechanisms underlying specific recruitment of CENP-A(Cnp1)/H4:Scm3sp into centromeres.
Mis16 Independently Recognizes Histone H4 and the CENP-ACnp1-Specific Chaperone Scm3sp.,An S, Kim H, Cho US J Mol Biol. 2015 Oct 9;427(20):3230-40. doi: 10.1016/j.jmb.2015.08.022. Epub 2015, Sep 4. PMID:26343758[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ An S, Kim H, Cho US. Mis16 Independently Recognizes Histone H4 and the CENP-ACnp1-Specific Chaperone Scm3sp. J Mol Biol. 2015 Oct 9;427(20):3230-40. doi: 10.1016/j.jmb.2015.08.022. Epub 2015, Sep 4. PMID:26343758 doi:http://dx.doi.org/10.1016/j.jmb.2015.08.022
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