1g6t

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[[Image:1g6t.jpg|left|200px]]
 
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{{Structure
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==STRUCTURE OF EPSP SYNTHASE LIGANDED WITH SHIKIMATE-3-PHOSPHATE==
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|PDB= 1g6t |SIZE=350|CAPTION= <scene name='initialview01'>1g6t</scene>, resolution 1.60&Aring;
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<StructureSection load='1g6t' size='340' side='right'caption='[[1g6t]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene>
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<table><tr><td colspan='2'>[[1g6t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G6T FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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|GENE= AROA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6t OCA], [https://pdbe.org/1g6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g6t RCSB], [https://www.ebi.ac.uk/pdbsum/1g6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g6t ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1g6s|1G6S]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6t OCA], [http://www.ebi.ac.uk/pdbsum/1g6t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1g6t RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/AROA_ECOLI AROA_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g6/1g6t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g6t ConSurf].
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<div style="clear:both"></div>
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'''STRUCTURE OF EPSP SYNTHASE LIGANDED WITH SHIKIMATE-3-PHOSPHATE'''
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==See Also==
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*[[EPSP synthase 3D structures|EPSP synthase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Biosynthesis of aromatic amino acids in plants, many bacteria, and microbes relies on the enzyme 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, a prime target for drugs and herbicides. We have identified the interaction of EPSP synthase with one of its two substrates (shikimate 3-phosphate) and with the widely used herbicide glyphosate by x-ray crystallography. The two-domain enzyme closes on ligand binding, thereby forming the active site in the interdomain cleft. Glyphosate appears to occupy the binding site of the second substrate of EPSP synthase (phosphoenol pyruvate), mimicking an intermediate state of the ternary enzyme.substrates complex. The elucidation of the active site of EPSP synthase and especially of the binding pattern of glyphosate provides a valuable roadmap for engineering new herbicides and herbicide-resistant crops, as well as new antibiotic and antiparasitic drugs.
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==About this Structure==
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1G6T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6T OCA].
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==Reference==
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Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail., Schonbrunn E, Eschenburg S, Shuttleworth WA, Schloss JV, Amrhein N, Evans JN, Kabsch W, Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1376-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11171958 11171958]
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[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Amrhein, N.]]
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[[Category: Amrhein N]]
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[[Category: Eschenburg, S.]]
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[[Category: Eschenburg S]]
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[[Category: Evans, J N.S.]]
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[[Category: Evans JNS]]
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[[Category: Kabsch, W.]]
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[[Category: Kabsch W]]
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[[Category: Schloss, J V.]]
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[[Category: Schloss JV]]
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[[Category: Schonbrunn, E.]]
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[[Category: Schonbrunn E]]
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[[Category: Shuttleworth, W.]]
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[[Category: Shuttleworth W]]
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[[Category: inside-out alpha-beta barrel]]
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[[Category: two-domain structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:37:47 2008''
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Current revision

STRUCTURE OF EPSP SYNTHASE LIGANDED WITH SHIKIMATE-3-PHOSPHATE

PDB ID 1g6t

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