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| - | [[Image:1g6u.jpg|left|200px]] | |
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| - | {{Structure
| + | ==CRYSTAL STRUCTURE OF A DOMAIN SWAPPED DIMER== |
| - | |PDB= 1g6u |SIZE=350|CAPTION= <scene name='initialview01'>1g6u</scene>, resolution 1.48Å
| + | <StructureSection load='1g6u' size='340' side='right'caption='[[1g6u]], [[Resolution|resolution]] 1.48Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TFA:TRIFLUOROACETYL+GROUP'>TFA</scene> | + | <table><tr><td colspan='2'>[[1g6u]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G6U FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.48Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TFA:TRIFLUOROACETIC+ACID'>TFA</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6u OCA], [https://pdbe.org/1g6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g6u RCSB], [https://www.ebi.ac.uk/pdbsum/1g6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g6u ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6u OCA], [http://www.ebi.ac.uk/pdbsum/1g6u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1g6u RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''CRYSTAL STRUCTURE OF A DOMAIN SWAPPED DIMER'''
| + | [[Category: Bryson JW]] |
| - | | + | [[Category: DeGrado WF]] |
| - | | + | [[Category: Eisenberg D]] |
| - | ==Overview==
| + | [[Category: Ghirlanda G]] |
| - | Three-dimensional (3D) domain-swapped proteins are intermolecularly folded analogs of monomeric proteins; both are stabilized by the identical interactions, but the individual domains interact intramolecularly in monomeric proteins, whereas they form intermolecular interactions in 3D domain-swapped structures. The structures and conditions of formation of several domain-swapped dimers and trimers are known, but the formation of higher order 3D domain-swapped oligomers has been less thoroughly studied. Here we contrast the structural consequences of domain swapping from two designed three-helix bundles: one with an up-down-up topology, and the other with an up-down-down topology. The up-down-up topology gives rise to a domain-swapped dimer whose structure has been determined to 1.5 A resolution by x-ray crystallography. In contrast, the domain-swapped protein with an up-down-down topology forms fibrils as shown by electron microscopy and dynamic light scattering. This demonstrates that design principles can predict the oligomeric state of 3D domain-swapped molecules, which should aid in the design of domain-swapped proteins and biomaterials.
| + | [[Category: Gingery M]] |
| - | | + | [[Category: Ogihara NL]] |
| - | ==About this Structure==
| + | |
| - | 1G6U is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6U OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Design of three-dimensional domain-swapped dimers and fibrous oligomers., Ogihara NL, Ghirlanda G, Bryson JW, Gingery M, DeGrado WF, Eisenberg D, Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1404-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11171963 11171963]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Bryson, J W.]] | + | |
| - | [[Category: DeGrado, W F.]] | + | |
| - | [[Category: Eisenberg, D.]] | + | |
| - | [[Category: Ghirlanda, G.]] | + | |
| - | [[Category: Gingery, M.]] | + | |
| - | [[Category: Ogihara, N L.]] | + | |
| - | [[Category: designed three helix bundle]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:37:47 2008''
| + | |
