5c22

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==Crystal structure of Zn-bound HlyD from E. coli==
==Crystal structure of Zn-bound HlyD from E. coli==
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<StructureSection load='5c22' size='340' side='right' caption='[[5c22]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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<StructureSection load='5c22' size='340' side='right'caption='[[5c22]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5c22]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C22 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C22 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5c22]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C22 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.302&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5c21|5c21]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c22 OCA], [http://pdbe.org/5c22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c22 RCSB], [http://www.ebi.ac.uk/pdbsum/5c22 PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c22 OCA], [https://pdbe.org/5c22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c22 RCSB], [https://www.ebi.ac.uk/pdbsum/5c22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c22 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/HLYDC_ECOLX HLYDC_ECOLX] Involved in the transport of hemolysin A.
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The protein toxin HlyA of Escherichia coli is exported without a periplasmic intermediate by the type I secretion system (T1SS). The T1SS is composed of an inner membrane ABC transporter HlyB, an outer-membrane channel protein TolC, and a membrane fusion protein HlyD. However, the assembly of the T1SS remains to be elucidated. In this study, we determine the crystal structure of a part of the C-terminal periplasmic domain of HlyD. The long alpha-helical domain consisting of three alpha helices and a lipoyl domain was identified in the crystal structure. Based on the HlyD structure, we modeled the hexameric assembly of HlyD with a long alpha-helical barrel, which formed a complex with TolC in an intermeshing cogwheel-to-cogwheel manner, as observed in tripartite RND-type drug efflux pumps. These observations provide a structural blueprint for understanding the type I secretion system in pathogenic Gram-negative bacteria.
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Crystal Structure of a Soluble Fragment of the Membrane Fusion Protein HlyD in a Type I Secretion System of Gram-Negative Bacteria.,Kim JS, Song S, Lee M, Lee S, Lee K, Ha NC Structure. 2016 Jan 22. pii: S0969-2126(16)00005-8. doi:, 10.1016/j.str.2015.12.012. PMID:26833388<ref>PMID:26833388</ref>
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==See Also==
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*[[Hemolysin 3D structures|Hemolysin 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5c22" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Ha, N C]]
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[[Category: Escherichia coli]]
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[[Category: Kim, J S]]
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[[Category: Large Structures]]
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[[Category: Hlyd]]
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[[Category: Ha NC]]
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[[Category: Multicrystal averaging]]
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[[Category: Kim JS]]
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[[Category: Protein transport]]
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[[Category: Type i secretion system]]
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[[Category: Zn sad]]
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Current revision

Crystal structure of Zn-bound HlyD from E. coli

PDB ID 5c22

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