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Nisin
From Proteopedia
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| - | <StructureSection load='1wco' size='340' side='right' caption='60/607847/Nisin_structure/4' scene=''> | + | <StructureSection load='1wco' size='340' side='right' caption='60/607847/Nisin_structure/4 in complex with lipid (PDB code [[1wco]])' scene=''> |
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== Function == | == Function == | ||
| - | + | '''Nisin''' is a polycylic antibacterial peptide, with 34 amino acid residues used as a food preservative. | |
Nisin is produced by fermentation using the bacterium ''Lactococcus lactis''. | Nisin is produced by fermentation using the bacterium ''Lactococcus lactis''. | ||
| - | While in general most | + | While in general most bacteriocins inhibit only closely related species, nisin is a rare example of a "broad-spectrum" bacteriocin effective against many Gram-positive organisms, including lactic acid bacteria (commonly associated with spoilage), ''Listeria monocytogenes'' (a known pathogen), ''Staphylococcus aureus,'' ''Bacillus cereus'', ''Clostridium botulinum'', etc. It is also particularly effective against spores. Gram-negative bacteria are protected by their outer membrane but may become susceptible to nisin action after a heat shock or when this is coupled with the chelator EDTA. Nisin is soluble in water and can be effective at levels nearing the parts-per-billion range<ref>PMID:2119570</ref>. |
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== Relevance == | == Relevance == | ||
| - | In the food industry, it is obtained from the culturing of ''L. lactis'' on natural substrates, such as milk or dextrose, and is not chemically synthesized. | + | In the food industry, it is obtained from the culturing of ''L. lactis'' on natural substrates, such as milk or dextrose, and is not chemically synthesized. Nisin is studied as a novel antibiotic due to its antimicrobial activity and as a therapeutic against neck and head squamous cell carcinoma<ref>PMID:26132406</ref>. |
== Structural highlights == | == Structural highlights == | ||
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</StructureSection> | </StructureSection> | ||
== 3D structure of nisin == | == 3D structure of nisin == | ||
| + | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | [[ | + | [[5xhb]] - LlNisin - ''Lactococcus lactis'' <br /> |
| + | [[1wco]] - LlNisin + lipid - NMR<br /> | ||
| + | [[2n32]] - LlNisin N-terminal - NMR<br /> | ||
| + | [[2n2e]] - LlNisin C-terminal - NMR<br /> | ||
== References == | == References == | ||
<references/> | <references/> | ||
| + | [[Category:Topic Page]] | ||
Current revision
Nisin
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3D structure of nisin
Updated on 22-November-2021
5xhb - LlNisin - Lactococcus lactis
1wco - LlNisin + lipid - NMR
2n32 - LlNisin N-terminal - NMR
2n2e - LlNisin C-terminal - NMR
References
- ↑ Liu W, Hansen JN. Some chemical and physical properties of nisin, a small-protein antibiotic produced by Lactococcus lactis. Appl Environ Microbiol. 1990 Aug;56(8):2551-8. PMID:2119570
- ↑ Kamarajan P, Hayami T, Matte B, Liu Y, Danciu T, Ramamoorthy A, Worden F, Kapila S, Kapila Y. Nisin ZP, a Bacteriocin and Food Preservative, Inhibits Head and Neck Cancer Tumorigenesis and Prolongs Survival. PLoS One. 2015 Jul 1;10(7):e0131008. doi: 10.1371/journal.pone.0131008., eCollection 2015. PMID:26132406 doi:http://dx.doi.org/10.1371/journal.pone.0131008
