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1gam

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[[Image:1gam.jpg|left|200px]]
 
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{{Structure
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==GAMMA B CRYSTALLIN TRUNCATED C-TERMINAL DOMAIN==
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|PDB= 1gam |SIZE=350|CAPTION= <scene name='initialview01'>1gam</scene>, resolution 2.6&Aring;
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<StructureSection load='1gam' size='340' side='right'caption='[[1gam]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1gam]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GAM FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gam OCA], [https://pdbe.org/1gam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gam RCSB], [https://www.ebi.ac.uk/pdbsum/1gam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gam ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gam OCA], [http://www.ebi.ac.uk/pdbsum/1gam PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gam RCSB]</span>
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[https://www.uniprot.org/uniprot/CRGB_BOVIN CRGB_BOVIN] Crystallins are the dominant structural components of the vertebrate eye lens.
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ga/1gam_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gam ConSurf].
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<div style="clear:both"></div>
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'''GAMMA B CRYSTALLIN TRUNCATED C-TERMINAL DOMAIN'''
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==See Also==
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*[[Crystallin 3D structures|Crystallin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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We use protein engineering and crystallography to simulate aspects of the early evolution of beta gamma-crystallins by observing how a single domain oligomerizes in response to changes in a sequence extension. The crystal structure of the C-terminal domain of gamma beta-crystallin with its four-residue C-terminal extension shows that the domain does not form a symmetric homodimer analogous to the two-domain pairing in beta gamma-crystallins. Instead the C-terminal extension now forms heterologous interactions with other domains leading to the solvent exposure of the natural hydrophobic interface with a consequent loss in protein solubility. However, this domain truncated by just the C-terminal tyrosine forms a symmetric homodimer of domains in the crystal lattice.
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==About this Structure==
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1GAM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GAM OCA].
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==Reference==
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The X-ray structures of two mutant crystallin domains shed light on the evolution of multi-domain proteins., Norledge BV, Mayr EM, Glockshuber R, Bateman OA, Slingsby C, Jaenicke R, Driessen HP, Nat Struct Biol. 1996 Mar;3(3):267-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8605629 8605629]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bateman, O A.]]
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[[Category: Bateman OA]]
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[[Category: Driessen, H P.C.]]
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[[Category: Driessen HPC]]
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[[Category: Glockshuber, R.]]
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[[Category: Glockshuber R]]
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[[Category: Jaenicke, R.]]
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[[Category: Jaenicke R]]
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[[Category: Mayr, E M.]]
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[[Category: Mayr E-M]]
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[[Category: Norledge, B V.]]
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[[Category: Norledge BV]]
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[[Category: Slingsby, C.]]
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[[Category: Slingsby C]]
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[[Category: eye lens protein]]
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[[Category: gamma crystallin b]]
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[[Category: multigene family]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:40:11 2008''
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Current revision

GAMMA B CRYSTALLIN TRUNCATED C-TERMINAL DOMAIN

PDB ID 1gam

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