5djn

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:09, 20 March 2024) (edit) (undo)
 
(2 intermediate revisions not shown.)
Line 1: Line 1:
==Crystal structure of the Kinesin-3 KIF13A NC-CC1 mutant - Deletion of P390==
==Crystal structure of the Kinesin-3 KIF13A NC-CC1 mutant - Deletion of P390==
-
<StructureSection load='5djn' size='340' side='right' caption='[[5djn]], [[Resolution|resolution]] 2.82&Aring;' scene=''>
+
<StructureSection load='5djn' size='340' side='right'caption='[[5djn]], [[Resolution|resolution]] 2.82&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[5djn]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DJN FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[5djn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DJN FirstGlance]. <br>
-
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5djn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5djn OCA], [http://pdbe.org/5djn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5djn RCSB], [http://www.ebi.ac.uk/pdbsum/5djn PDBsum]</span></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.82&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5djn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5djn OCA], [https://pdbe.org/5djn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5djn RCSB], [https://www.ebi.ac.uk/pdbsum/5djn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5djn ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/F8VQ75_MOUSE F8VQ75_MOUSE]
-
Processive kinesin motors often contain a coiled-coil neck that controls the directionality and processivity. However, the neck coil (NC) of kinesin-3 is too short to form a stable coiled-coil dimer. Here, we found that the CC1-FHA tandem (that is connected to NC by P390) of kinesin-3 KIF13A assembles as an extended dimer. With the removal of P390, the NC-CC1 tandem of KIF13A unexpectedly forms a continuous coiled-coil dimer that can be well aligned into the CC1-FHA dimer. The reverse introduction of P390 breaks the NC-CC1 coiled-coil dimer but provides the intrinsic flexibility to couple NC with the CC1-FHA tandem. Mutations of either NC, CC1 or the FHA domain all significantly impaired the motor activity. Thus, the three elements within the NC-CC1-FHA tandem of KIF13A are structurally interrelated to form a stable dimer for activating the motor. This work also provides the first direct structural evidence to support the formation of a coiled-coil neck by the short characteristic neck domain of kinesin-3.
+
-
Structural correlation of the neck coil with the CC1-FHA tandem for active kinesin-3 KIF13A.,Ren J, Huo L, Wang W, Zhang Y, Li W, Lou J, Xu T, Feng W J Biol Chem. 2015 Dec 17. pii: jbc.M115.689091. PMID:26680000<ref>PMID:26680000</ref>
+
==See Also==
-
 
+
*[[Kinesin 3D Structures|Kinesin 3D Structures]]
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 5djn" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Feng, W]]
+
[[Category: Large Structures]]
-
[[Category: Ren, J Q]]
+
[[Category: Mus musculus]]
-
[[Category: Coil-coil]]
+
[[Category: Feng W]]
-
[[Category: Transport protein]]
+
[[Category: Ren JQ]]

Current revision

Crystal structure of the Kinesin-3 KIF13A NC-CC1 mutant - Deletion of P390

PDB ID 5djn

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5djn"
Personal tools