5i69
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==MBP-MamC magnetite-interaction component mutant-D70A== | |
| + | <StructureSection load='5i69' size='340' side='right'caption='[[5i69]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5i69]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Magnetospirillum_magneticum_AMB-1 Magnetospirillum magneticum AMB-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I69 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I69 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i69 OCA], [https://pdbe.org/5i69 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i69 RCSB], [https://www.ebi.ac.uk/pdbsum/5i69 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i69 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/MAMC_MAGSA MAMC_MAGSA] Probably involved in magnetite crystal growth (Probable). The lumenal domain may bind the magnetite crystals, affecting crystal size and shape (Probable).<ref>PMID:24961165</ref> <ref>PMID:26970040</ref> <ref>PMID:30405554</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Magnetotactic bacteria are Gram-negative bacteria that navigate along geomagnetic fields using the magnetosome, an organelle that consists of a membrane-enveloped magnetic nanoparticle. Magnetite formation and its properties are controlled by a specific set of proteins. MamC is a small magnetosome-membrane protein that is known to be active in iron biomineralization but its mechanism has yet to be clarified. Here, we studied the relationship between the MamC magnetite-interaction loop (MIL) structure and its magnetite interaction using an inert biomineralization protein-MamC chimera. Our determined structure shows an alpha-helical fold for MamC-MIL with highly charged surfaces. Additionally, the MamC-MIL induces the formation of larger magnetite crystals compared to protein-free and inert biomineralization protein control experiments. We suggest that the connection between the MamC-MIL structure and the protein's charged surfaces is crucial for magnetite binding and thus for the size control of the magnetite nanoparticles. | ||
| - | + | Structure-function studies of the magnetite-biomineralizing magnetosome-associated protein MamC.,Nudelman H, Valverde-Tercedor C, Kolusheva S, Perez Gonzalez T, Widdrat M, Grimberg N, Levi H, Nelkenbaum O, Davidov G, Faivre D, Jimenez-Lopez C, Zarivach R J Struct Biol. 2016 Jun;194(3):244-52. doi: 10.1016/j.jsb.2016.03.001. Epub 2016 , Mar 10. PMID:26970040<ref>PMID:26970040</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5i69" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Jimenez-Lopez | + | </StructureSection> |
| - | [[Category: Kolusheva | + | [[Category: Escherichia coli]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Magnetospirillum magneticum AMB-1]] |
| - | [[Category: | + | [[Category: Davidove G]] |
| - | [[Category: | + | [[Category: Faivre D]] |
| - | [[Category: | + | [[Category: Gonzalez TP]] |
| - | [[Category: | + | [[Category: Grimberg N]] |
| + | [[Category: Jimenez-Lopez C]] | ||
| + | [[Category: Kolusheva S]] | ||
| + | [[Category: Levi H]] | ||
| + | [[Category: Nelkenbaum O]] | ||
| + | [[Category: Nudelman H]] | ||
| + | [[Category: Tercedor CV]] | ||
| + | [[Category: Widdrat M]] | ||
| + | [[Category: Zarivach R]] | ||
Current revision
MBP-MamC magnetite-interaction component mutant-D70A
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