5epv

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==Histidine kinase domain from the LOV-HK blue-light receptor from Brucella abortus==
==Histidine kinase domain from the LOV-HK blue-light receptor from Brucella abortus==
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<StructureSection load='5epv' size='340' side='right' caption='[[5epv]], [[Resolution|resolution]] 2.51&Aring;' scene=''>
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<StructureSection load='5epv' size='340' side='right'caption='[[5epv]], [[Resolution|resolution]] 2.51&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5epv]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EPV FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5epv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Brucella_abortus_2308 Brucella abortus 2308]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EPV FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.51&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3t50|3t50]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5epv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5epv OCA], [https://pdbe.org/5epv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5epv RCSB], [https://www.ebi.ac.uk/pdbsum/5epv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5epv ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5epv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5epv OCA], [http://pdbe.org/5epv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5epv RCSB], [http://www.ebi.ac.uk/pdbsum/5epv PDBsum]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/LOVHK_BRUA2 LOVHK_BRUA2]] Photosensitive kinase that is involved in increased bacterial virulence upon exposure to light. Once ejected from an infected animal host, sunlight acts as an environmental signal that increases the virulence of the bacterium, preparing it for infection of the next host (By similarity). This photoreceptor protein is directly related to the bacterium's survival and replication within host macrophages, as it is required for optimal replication of bacteria inside macrophages.<ref>PMID:17717187</ref>
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[https://www.uniprot.org/uniprot/LOVHK_BRUA2 LOVHK_BRUA2] Photosensitive kinase that is involved in increased bacterial virulence upon exposure to light. Once ejected from an infected animal host, sunlight acts as an environmental signal that increases the virulence of the bacterium, preparing it for infection of the next host (By similarity). This photoreceptor protein is directly related to the bacterium's survival and replication within host macrophages, as it is required for optimal replication of bacteria inside macrophages.<ref>PMID:17717187</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In response to light, as part of a two-component system, the Brucella blue light-activated histidine kinase (LOV-HK) increases its autophosphorylation, modulating the virulence of this microorganism. The Brucella histidine kinase (HK) domain belongs to the HWE family, for which there is no structural information. The HWE family is exclusively present in proteobacteria and usually coupled to a wide diversity of light sensor domains. This work reports the crystal structure of the Brucella HK domain, which presents two different dimeric assemblies in the asymmetric unit: one similar to the already described canonical parallel homodimers (C) and the other, an antiparallel non-canonical (NC) dimer, each with distinct relative subdomain orientations and dimerization interfaces. Contrary to these crystallographic structures and unlike other HKs, in solution, the Brucella HK domain is monomeric and still active, showing an astonishing instability of the dimeric interface. Despite this instability, using cross-linking experiments, we show that the C dimer is the functionally relevant species. Mutational analysis demonstrates that the autophosphorylation activity occurs in cis. The different relative subdomain orientations observed for the NC and C states highlight the large conformational flexibility of the HK domain. Through the analysis of these alternative conformations by means of molecular dynamics simulations, we also propose a catalytic mechanism for Brucella LOV-HK.
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Structural Insights into the HWE Histidine Kinase Family: The Brucella Blue Light-Activated Histidine Kinase Domain.,Rinaldi J, Arrar M, Sycz G, Cerutti ML, Berguer PM, Paris G, Estrin DA, Marti MA, Klinke S, Goldbaum FA J Mol Biol. 2016 Feb 2. pii: S0022-2836(16)00081-4. doi:, 10.1016/j.jmb.2016.01.026. PMID:26851072<ref>PMID:26851072</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5epv" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Histidine kinase]]
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[[Category: Brucella abortus 2308]]
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[[Category: Goldbaum, F A]]
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[[Category: Large Structures]]
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[[Category: Guimaraes, B G]]
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[[Category: Goldbaum FA]]
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[[Category: Klinke, S]]
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[[Category: Guimaraes BG]]
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[[Category: Legrand, P]]
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[[Category: Klinke S]]
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[[Category: Paris, G]]
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[[Category: Legrand P]]
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[[Category: Rinaldi, J]]
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[[Category: Paris G]]
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[[Category: Thompson, A]]
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[[Category: Rinaldi J]]
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[[Category: Hwe family]]
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[[Category: Thompson A]]
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[[Category: Multi-crystal data collection]]
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[[Category: S-sad phasing]]
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[[Category: Transferase]]
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Current revision

Histidine kinase domain from the LOV-HK blue-light receptor from Brucella abortus

PDB ID 5epv

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