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| | ==Solution NMR structure of Contryphan-Vc1== | | ==Solution NMR structure of Contryphan-Vc1== |
| - | <StructureSection load='2n24' size='340' side='right' caption='[[2n24]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2n24' size='340' side='right'caption='[[2n24]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2n24]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N24 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N24 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2n24]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_victoriae Conus victoriae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N24 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N24 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n24 OCA], [http://pdbe.org/2n24 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n24 RCSB], [http://www.ebi.ac.uk/pdbsum/2n24 PDBsum]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n24 OCA], [https://pdbe.org/2n24 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n24 RCSB], [https://www.ebi.ac.uk/pdbsum/2n24 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n24 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/O2VC1_CONVC O2VC1_CONVC] Unknown. Intracranial injection of the peptide into mice does not produce toxic effects (PubMed:26774129). In addition, the peptide does not produce any observable changes to normal or depolarization-induced intracellular calcium levels in mouse dorsal root ganglion cells (PubMed:26774129).<ref>PMID:26774129</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chhabra, S]] | + | [[Category: Conus victoriae]] |
| - | [[Category: Norton, R S]] | + | [[Category: Large Structures]] |
| - | [[Category: Robinson, S D]] | + | [[Category: Chhabra S]] |
| - | [[Category: Contryphan-vc1]] | + | [[Category: Norton RS]] |
| - | [[Category: Sdh]] | + | [[Category: Robinson SD]] |
| - | [[Category: Single disulfide-directed beta hairpin]]
| + | |
| - | [[Category: Toxin]]
| + | |
| Structural highlights
Function
O2VC1_CONVC Unknown. Intracranial injection of the peptide into mice does not produce toxic effects (PubMed:26774129). In addition, the peptide does not produce any observable changes to normal or depolarization-induced intracellular calcium levels in mouse dorsal root ganglion cells (PubMed:26774129).[1]
Publication Abstract from PubMed
Certain peptide folds, owing to a combination of intrinsic stability and resilience to amino acid substitutions, are particularly effective for the display of diverse functional groups. Such "privileged scaffolds" are valuable as starting points for the engineering of new bioactive molecules. We have identified a precursor peptide expressed in the venom gland of the marine snail Conus victoriae, which appears to belong to a hitherto undescribed class of molluscan neuropeptides. Mass spectrometry matching with the venom confirmed the complete mature peptide sequence as a 31-residue peptide with a single disulfide bond. Solution structure determination revealed a unique peptide fold that we have designated the single disulfide-directed beta hairpin (SDH). The SDH fold is highly resistant to thermal denaturation and forms the core of several other multiple disulfide-containing peptide folds, including the inhibitor cystine knot. This elementary fold may offer a valuable starting point for the design and engineering of new bioactive peptides.
A Naturally Occurring Peptide with an Elementary Single Disulfide-Directed beta-Hairpin Fold.,Robinson SD, Chhabra S, Belgi A, Chittoor B, Safavi-Hemami H, Robinson AJ, Papenfuss AT, Purcell AW, Norton RS Structure. 2016 Jan 2. pii: S0969-2126(15)00504-3. doi:, 10.1016/j.str.2015.11.015. PMID:26774129[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Robinson SD, Chhabra S, Belgi A, Chittoor B, Safavi-Hemami H, Robinson AJ, Papenfuss AT, Purcell AW, Norton RS. A Naturally Occurring Peptide with an Elementary Single Disulfide-Directed beta-Hairpin Fold. Structure. 2016 Jan 2. pii: S0969-2126(15)00504-3. doi:, 10.1016/j.str.2015.11.015. PMID:26774129 doi:http://dx.doi.org/10.1016/j.str.2015.11.015
- ↑ Robinson SD, Chhabra S, Belgi A, Chittoor B, Safavi-Hemami H, Robinson AJ, Papenfuss AT, Purcell AW, Norton RS. A Naturally Occurring Peptide with an Elementary Single Disulfide-Directed beta-Hairpin Fold. Structure. 2016 Jan 2. pii: S0969-2126(15)00504-3. doi:, 10.1016/j.str.2015.11.015. PMID:26774129 doi:http://dx.doi.org/10.1016/j.str.2015.11.015
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