This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1gjw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:21, 27 March 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1gjw.jpg|left|200px]]
 
-
{{Structure
+
==Thermotoga maritima maltosyltransferase complex with maltose==
-
|PDB= 1gjw |SIZE=350|CAPTION= <scene name='initialview01'>1gjw</scene>, resolution 2.1&Aring;
+
<StructureSection load='1gjw' size='340' side='right'caption='[[1gjw]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-
|SITE= <scene name='pdbsite=GLC:Glc+Binding+Site+For+Chain+A'>GLC</scene> and <scene name='pdbsite=MAL:Mal+Binding+Site+For+Chain+A'>MAL</scene>
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
+
<table><tr><td colspan='2'>[[1gjw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GJW FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gjw OCA], [https://pdbe.org/1gjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gjw RCSB], [https://www.ebi.ac.uk/pdbsum/1gjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gjw ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gjw OCA], [http://www.ebi.ac.uk/pdbsum/1gjw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gjw RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/O33838_THEMT O33838_THEMT]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gj/1gjw_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gjw ConSurf].
 +
<div style="clear:both"></div>
-
'''THERMOTOGA MARITIMA MALTOSYLTRANSFERASE COMPLEX WITH MALTOSE'''
+
==See Also==
-
 
+
*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
Maltosyltransferase (MTase) from the hyperthermophile Thermotoga maritima represents a novel maltodextrin glycosyltransferase acting on starch and malto-oligosaccharides. It catalyzes the transfer of maltosyl units from alpha-1,4-linked glucans or malto-oligosaccharides to other alpha-1,4-linked glucans, malto-oligosaccharides or glucose. It belongs to the glycoside hydrolase family 13, which represents a large group of (beta/alpha)(8) barrel proteins sharing a similar active site structure. The crystal structures of MTase and its complex with maltose have been determined at 2.4 A and 2.1 A resolution, respectively. MTase is a homodimer, each subunit of which consists of four domains, two of which are structurally homologous to those of other family 13 enzymes. The catalytic core domain has the (beta/alpha)(8) barrel fold with the active-site cleft formed at the C-terminal end of the barrel. Substrate binding experiments have led to the location of two distinct maltose-binding sites; one lies in the active-site cleft, covering subsites -2 and -1; the other is located in a pocket adjacent to the active-site cleft. The structure of MTase, together with the conservation of active-site residues among family 13 glycoside hydrolases, are consistent with a common double-displacement catalytic mechanism for this enzyme. Analysis of maltose binding in the active site reveals that the transfer of dextrinyl residues longer than a maltosyl unit is prevented by termination of the active-site cleft after the -2 subsite by the side-chain of Lys151 and the stretch of residues 314-317, providing an explanation for the strict transfer specificity of MTase.
+
[[Category: Large Structures]]
-
 
+
[[Category: Thermotoga maritima MSB8]]
-
==About this Structure==
+
[[Category: Baker PJ]]
-
1GJW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJW OCA].
+
[[Category: Burke J]]
-
 
+
[[Category: Liebl W]]
-
==Reference==
+
[[Category: Raasch C]]
-
The crystal structure of Thermotoga maritima maltosyltransferase and its implications for the molecular basis of the novel transfer specificity., Roujeinikova A, Raasch C, Burke J, Baker PJ, Liebl W, Rice DW, J Mol Biol. 2001 Sep 7;312(1):119-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11545590 11545590]
+
[[Category: Rice DW]]
-
[[Category: Single protein]]
+
[[Category: Roujeinikova A]]
-
[[Category: Thermotoga maritima]]
+
-
[[Category: Baker, P J.]]
+
-
[[Category: Burke, J.]]
+
-
[[Category: Liebl, W.]]
+
-
[[Category: Raasch, C.]]
+
-
[[Category: Rice, D W.]]
+
-
[[Category: Roujeinikova, A.]]
+
-
[[Category: alpha-amylase]]
+
-
[[Category: maltosyltransferase]]
+
-
[[Category: transferase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:45:38 2008''
+

Current revision

Thermotoga maritima maltosyltransferase complex with maltose

PDB ID 1gjw

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1gjw"
Personal tools