Gelsolin

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[[Image:3ffn.jpg|left|300px]]
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<StructureSection load='1h1v' size='350' side='right' caption='Human gelsolin S4-S6 (gold) complex with actin (cyan), ATP and Ca+2 ion (green) (PDB entry [[1h1v]])' scene='41/410297/Cv/3'>
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<!--
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__TOC__
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{{Structure
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|PDB=3ffn|SIZE=350|SCENE=|CAPTION=Gelsolin (GLS), 3FFN
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE=
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|DOMAIN=
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|RELATEDENTRY=
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|RESOURCES=
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}}
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<StructureSection load='1h1v' size='350' side='right' caption='Human gelsolin G4-G6 (gold) complex with actin (grey), ATP (stick model) and Ca+2 ion (green) (PDB entry [[1h1v]])' scene=''>
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== Function ==
== Function ==
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[[Gelsolin]] (GLS) is a protein regulator of actin assembly and disassembly<ref>PMID:10559185</ref>.
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[[Gelsolin]] (GLS) is a protein regulator of actin assembly and disassembly<ref>PMID:10559185</ref>. Binding of Ca+2 ion to GLS induces conformational change which enables it to bind actin.
== Structural highlights ==
== Structural highlights ==
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The image at the top left corresponds to the human gelsolin ([[3ffn]]), it has <scene name='Gelsolin/Cv/2'>dimeric form</scene>. It contains 6 homologous domains S1 to S6.
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GLS contains 6 homologous domains S1 to S6. The human GLS domains span residues: 15-135, 136-248, 249-367, 394-513, 514-619 and 620-734. <scene name='41/410297/Cv/6'>S4-S6 are shown</scene>. The <scene name='41/410297/Cv/7'>binding site of Ca+2 ion includes Glu, Asp and 2 carbonyl oxygens</scene><ref>PMID:12460571</ref>.
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== 3D Structures of GLS ==
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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{{#tree:id=OrganizedByTopic|openlevels=0|
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* GLS
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**[[3ffn]] – hGLS – human <br />
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**[[2fgh]] – hoGLS+ATP – horse <br />
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**[[1d0n]] - hoGLS <br />
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**[[2x1o]], [[2x1p]], [[2x1q]] – GLS nanobody - Llama
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* GLS domains S1-S3
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**[[3ffk]] – hGLS +actin <br />
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**[[1rgi]] - hoGLS +actin <br />
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* GLS domains S4-S6
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**[[1h1v]] - hGLS +actin<br />
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**[[1nph]] - mGLS +Ca – mouse<br />
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* GLS domain S1
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**[[2ff3]] - hGLS +N-WASP+actin<br />
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== 3D Structures of gelsolin ==
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**[[2ff6]] - hGLS +Ciboulot domain 2+actin<br />
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[[Gelsolin 3D structures]]
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**[1mdu]] - hGLS +chActin – chicken<br />
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**[[1d4x]] - hGLS +CeActin+Mg-ATP – ''Caenorhabditis elegans''<br />
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**[[1esv]] - hGLS +rActin+latrunculin – rabbit<br />
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**[[1eqy]] - hGLS +rActin<br />
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**[[1yvn]] - hGLS +yActin (mutant) – yeast<br />
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**[[1yag]] - hGLS +yActin<br />
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**[[1t44]] - hGLS/thymosin β-4 C-terminal fusion+rActin<br />
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**[[1kcq]] – hGLS +Cd<br />
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**[[1c0f]], [[1mdu]], [[1nmd]] - hGLS + Actin+Ca-ATP <br />
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**[[1c0g]], [[1dej]] - hGLS +Actin (mutant)<br />
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**[[3a5l]], [[3a5m]], [[3a5n]], [[3a5o]] - hGLS (mutant)+Actin (mutant)<br />
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**[[3ci5]], [[3cip]], [[1nlv]] – DdGLS +actin - ''Dictyostelium discoideum''<br />
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**[[1nm1]] - DdGLS +actin+Mg-ATP<br />
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**[[3cjb]] - rGLS +rActin<br />
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**[[3cjc]] - rGLS +rActin+DNAse I<br />
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**[[4cbu]], [[4cbw]], [[4cbx]] - mGLS +actin+Ca+ATP<br />
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* GLS C-terminal
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</StructureSection>
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**[[2fh1]], [[2fh2]], [[2fh3]], [[2fh4]] – hGLS <br />
 
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**[[1p8x]] - hGLS +Ca<br />
 
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**[[1p8z]] – hGLS fraction+rActin<br />
 
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}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Human gelsolin S4-S6 (gold) complex with actin (cyan), ATP and Ca+2 ion (green) (PDB entry 1h1v)

Drag the structure with the mouse to rotate

References

  1. ↑ Sun HQ, Yamamoto M, Mejillano M, Yin HL. Gelsolin, a multifunctional actin regulatory protein. J Biol Chem. 1999 Nov 19;274(47):33179-82. PMID:10559185
  2. ↑ Choe H, Burtnick LD, Mejillano M, Yin HL, Robinson RC, Choe S. The calcium activation of gelsolin: insights from the 3A structure of the G4-G6/actin complex. J Mol Biol. 2002 Dec 6;324(4):691-702. PMID:12460571

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Jaime Prilusky

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