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1gqz
From Proteopedia
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| - | [[Image:1gqz.gif|left|200px]] | ||
| - | + | ==Refinement of Haemophilus influenzae Diaminopimelate epimerase at 1.7A== | |
| - | + | <StructureSection load='1gqz' size='340' side='right'caption='[[1gqz]], [[Resolution|resolution]] 1.75Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[1gqz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae_Rd_KW20 Haemophilus influenzae Rd KW20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GQZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GQZ FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gqz OCA], [https://pdbe.org/1gqz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gqz RCSB], [https://www.ebi.ac.uk/pdbsum/1gqz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gqz ProSAT]</span></td></tr> | |
| - | + | </table> | |
| - | | | + | == Function == |
| - | | | + | [https://www.uniprot.org/uniprot/DAPF_HAEIN DAPF_HAEIN] Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan. Only accepts DAP isomers with the L configuration.<ref>PMID:10194362</ref> |
| - | + | == Evolutionary Conservation == | |
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gq/1gqz_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gqz ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Diaminopimelate (DAP) epimerase (DapF) is central to the biosynthesis of both lysine and cell-wall peptidoglycan in many bacteria species. The peptidoglycan layer provides great potential for the development of novel antimicrobials as it is a uniquely prokaryotic feature. Crystals of recombinant Haemophilus influenzae DapF that diffract to beyond 2 A resolution have been obtained which facilitated the solution of the structure by molecular replacement at a resolution approximately 1 A higher than that previously determined. An analysis of the structure (i) in comparison to other PLP-independent racemaces and (ii) in relation to the catalytic mechanism and stereospecificity of DapF is presented. | ||
| - | + | Refinement of Haemophilus influenzae diaminopimelic acid epimerase (DapF) at 1.75 A resolution suggests a mechanism for stereocontrol during catalysis.,Lloyd AJ, Huyton T, Turkenburg J, Roper DI Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):397-400. Epub 2004, Jan 23. PMID:14747737<ref>PMID:14747737</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1gqz" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | [[Category: Haemophilus influenzae]] | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Huyton | + | </StructureSection> |
| - | [[Category: Roper | + | [[Category: Haemophilus influenzae Rd KW20]] |
| - | [[Category: Turkenburg | + | [[Category: Large Structures]] |
| - | + | [[Category: Huyton T]] | |
| - | + | [[Category: Roper DI]] | |
| - | + | [[Category: Turkenburg JP]] | |
| - | + | ||
Current revision
Refinement of Haemophilus influenzae Diaminopimelate epimerase at 1.7A
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