5ie8
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The pyrazinoic acid binding domain of Ribosomal Protein S1 from Mycobacterium tuberculosis== |
| + | <StructureSection load='5ie8' size='340' side='right'caption='[[5ie8]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5ie8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_leprae_TN Mycobacterium leprae TN]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IE8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IE8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ie8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ie8 OCA], [https://pdbe.org/5ie8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ie8 RCSB], [https://www.ebi.ac.uk/pdbsum/5ie8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ie8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RS1_MYCLE RS1_MYCLE] Binds mRNA; thus facilitating recognition of the initiation point. It is needed to translate mRNA with a short Shine-Dalgarno (SD) purine-rich sequence (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Ribosomal protein S1 of Mycobacterium tuberculosis (MtRpsA) binds to ribosome and mRNA, and plays significant role in the regulation of translation initiation, conventional protein synthesis and transfer-messenger RNA (tmRNA) mediated trans-translation. It has been identified as the target of pyrazinoic acid (POA), a bactericidal moiety from hydrolysis of pyrazinamide, which is a mainstay of combination therapy for tuberculosis. POA prevented the interactions between the C-terminal S1 domain of MtRpsA (residues 280-368, MtRpsA(CTD)_S1) and tmRNA; so that POA can inhibit the trans-translation, which is a key component of multiple quality control pathways in bacteria. However, the details of molecular mechanism and dynamic characteristics for MtRpsA(CTD)_S1 interactions with POA, tmRNA or mRNA are still unclear. Here we present the (1)H, (15)N, (13)C resonance assignments of MtRpsA(CTD)_S1 as well as the secondary structure information based on backbone chemical shifts, which lay foundation for further solution structure determination, dynamic properties characterization and interactions investigation between MtRpsA(CTD)_S1 and tmRNA, RNA or POA. | ||
| - | + | (1)H, (15)N, (13)C resonance assignments for pyrazinoic acid binding domain of ribosomal protein S1 from Mycobacterium tuberculosis.,Huang B, Fu J, Guo C, Wu X, Lin D, Liao X Biomol NMR Assign. 2016 Oct;10(2):321-4. doi: 10.1007/s12104-016-9692-9. Epub, 2016 Jul 13. PMID:27412769<ref>PMID:27412769</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Huang | + | <div class="pdbe-citations 5ie8" style="background-color:#fffaf0;"></div> |
| - | [[Category: Liao | + | |
| + | ==See Also== | ||
| + | *[[Ribosomal protein S1|Ribosomal protein S1]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mycobacterium leprae TN]] | ||
| + | [[Category: Huang B]] | ||
| + | [[Category: Liao X]] | ||
Current revision
The pyrazinoic acid binding domain of Ribosomal Protein S1 from Mycobacterium tuberculosis
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