1gtq

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[[Image:1gtq.gif|left|200px]]
 
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{{Structure
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==6-PYRUVOYL TETRAHYDROPTERIN SYNTHASE==
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|PDB= 1gtq |SIZE=350|CAPTION= <scene name='initialview01'>1gtq</scene>, resolution 2.3&Aring;
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<StructureSection load='1gtq' size='340' side='right'caption='[[1gtq]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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<table><tr><td colspan='2'>[[1gtq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. The August 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Tetrahydrobiopterin Biosynthesis'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_8 10.2210/rcsb_pdb/mom_2015_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GTQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GTQ FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/6-pyruvoyltetrahydropterin_synthase 6-pyruvoyltetrahydropterin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.12 4.2.3.12] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gtq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gtq OCA], [https://pdbe.org/1gtq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gtq RCSB], [https://www.ebi.ac.uk/pdbsum/1gtq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gtq ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gtq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gtq OCA], [http://www.ebi.ac.uk/pdbsum/1gtq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gtq RCSB]</span>
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== Disease ==
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}}
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[https://www.uniprot.org/uniprot/PTPS_RAT PTPS_RAT] Note=Deficiency leads to phenylketonuria.
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== Function ==
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'''6-PYRUVOYL TETRAHYDROPTERIN SYNTHASE'''
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[https://www.uniprot.org/uniprot/PTPS_RAT PTPS_RAT] Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The crystal structure of rat liver 6-pyruvoyl tetrahydropterin synthase has been solved by multiple isomorphous replacement and refined to a crystallographic R-factor of 20.4% at 2.3 A resolution. 6-Pyruvoyl tetrahydrobiopterin synthase catalyses the conversion of dihydroneopterin triphosphate to 6-pyruvoyl tetrahydropterin, the second of three enzymatic steps in the synthesis of tetrahydrobiopterin from GTP. The functional enzyme is a hexamer of identical subunits. The 6-pyruvoyl tetrahydropterin synthase monomer folds into a sequential, four-stranded, antiparallel beta-sheet with a 25 residue, helix-containing insertion between strands 1 and 2 at the bottom of the molecule, and a segment between strands 2 and 3 forming a pair of antiparallel helices, layered on one side of the beta-sheet. Three 6-pyruvoyl tetrahydropterin synthase monomers form an unusual 12-stranded antiparallel beta-barrel by tight association between the N- and C-terminal beta-strands of two adjacent subunits. The barrel encloses a highly basic pore of 6-12 A diameter. Two trimers associate in a head-to-head fashion to form the active enzyme complex. The substrate-binding site is located close to the trimer-trimer interface and comprises residues from three monomers: A, A' and B. A metal-binding site in the substrate-binding pocket is formed by the three histidine residues 23, 48 and 50 from one 6-pyruvoyl tetrahydropterin synthase subunit. Close to the metal, but apparently not liganding it, are residues Cys42, Glu133 (both from A) and His89 (from B), which might serve as proton donors and acceptors during catalysis.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gt/1gtq_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1GTQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GTQ OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gtq ConSurf].
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Three-dimensional structure of 6-pyruvoyl tetrahydropterin synthase, an enzyme involved in tetrahydrobiopterin biosynthesis., Nar H, Huber R, Heizmann CW, Thony B, Burgisser D, EMBO J. 1994 Mar 15;13(6):1255-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8137809 8137809]
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<div style="clear:both"></div>
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[[Category: 6-pyruvoyltetrahydropterin synthase]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: Rattus rattus]]
[[Category: Rattus rattus]]
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[[Category: Single protein]]
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[[Category: Tetrahydrobiopterin Biosynthesis]]
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[[Category: Buergisser, D.]]
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[[Category: Buergisser D]]
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[[Category: Heizmann, C W.]]
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[[Category: Heizmann CW]]
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[[Category: Huber, R.]]
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[[Category: Huber R]]
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[[Category: Nar, H.]]
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[[Category: Nar H]]
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[[Category: Thoeny, B.]]
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[[Category: Thoeny B]]
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[[Category: phosphate elimination]]
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[[Category: pterine synthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:51:05 2008''
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Current revision

6-PYRUVOYL TETRAHYDROPTERIN SYNTHASE

PDB ID 1gtq

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