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3jbh

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==TWO HEAVY MEROMYOSIN INTERACTING-HEADS MOTIFS FLEXIBLE DOCKED INTO TARANTULA THICK FILAMENT 3D-MAP ALLOWS IN DEPTH STUDY OF INTRA- AND INTERMOLECULAR INTERACTIONS==
==TWO HEAVY MEROMYOSIN INTERACTING-HEADS MOTIFS FLEXIBLE DOCKED INTO TARANTULA THICK FILAMENT 3D-MAP ALLOWS IN DEPTH STUDY OF INTRA- AND INTERMOLECULAR INTERACTIONS==
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<StructureSection load='3jbh' size='340' side='right' caption='[[3jbh]], [[Resolution|resolution]] 20.00&Aring;' scene=''>
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<SX load='3jbh' size='340' side='right' viewer='molstar' caption='[[3jbh]], [[Resolution|resolution]] 20.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3jbh]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Aphonopelma Aphonopelma]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JBH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JBH FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3jbh]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Aphonopelma Aphonopelma]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JBH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JBH FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3dtp|3dtp]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 20&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jbh OCA], [http://pdbe.org/3jbh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jbh RCSB], [http://www.ebi.ac.uk/pdbsum/3jbh PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jbh OCA], [https://pdbe.org/3jbh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jbh RCSB], [https://www.ebi.ac.uk/pdbsum/3jbh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jbh ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A140UGH3_9ARAC A0A140UGH3_9ARAC]
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Tarantula striated muscle is an outstanding system for understanding the molecular organization of myosin filaments. Three-dimensional reconstruction based on cryo-electron microscopy images and single-particle image processing revealed that, in a relaxed state, myosin molecules undergo intramolecular head-head interactions, explaining why head activity switches off. The filament model obtained by rigidly docking a chicken smooth muscle myosin structure to the reconstruction was improved by flexibly fitting an atomic model built by mixing structures from different species to a tilt-corrected 2-nm three-dimensional map of frozen-hydrated tarantula thick filament. We used heavy and light chain sequences from tarantula myosin to build a single-species homology model of two heavy meromyosin interacting-heads motifs (IHMs). The flexibly fitted model includes previously missing loops and shows five intramolecular and five intermolecular interactions that keep the IHM in a compact off structure, forming four helical tracks of IHMs around the backbone. The residues involved in these interactions are oppositely charged, and their sequence conservation suggests that IHM is present across animal species. The new model, PDB 3JBH, explains the structural origin of the ATP turnover rates detected in relaxed tarantula muscle by ascribing the very slow rate to docked unphosphorylated heads, the slow rate to phosphorylated docked heads, and the fast rate to phosphorylated undocked heads. The conservation of intramolecular interactions across animal species and the presence of IHM in bilaterians suggest that a super-relaxed state should be maintained, as it plays a role in saving ATP in skeletal, cardiac, and smooth muscles.
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Conserved Intramolecular Interactions Maintain Myosin Interacting-Heads Motifs Explaining Tarantula Muscle Super-Relaxed State Structural Basis.,Alamo L, Qi D, Wriggers W, Pinto A, Zhu J, Bilbao A, Gillilan RE, Hu S, Padron R J Mol Biol. 2016 Feb 2. pii: S0022-2836(16)00082-6. doi:, 10.1016/j.jmb.2016.01.027. PMID:26851071<ref>PMID:26851071</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3jbh" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
[[Category: Aphonopelma]]
[[Category: Aphonopelma]]
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[[Category: Alamo, L]]
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[[Category: Large Structures]]
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[[Category: Bilbao, A]]
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[[Category: Alamo L]]
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[[Category: Gillilan, R E]]
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[[Category: Bilbao A]]
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[[Category: Hu, S]]
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[[Category: Gillilan RE]]
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[[Category: Padron, R]]
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[[Category: Hu S]]
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[[Category: Pinto, A]]
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[[Category: Padron R]]
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[[Category: Qi, D]]
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[[Category: Pinto A]]
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[[Category: Wriggers, W]]
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[[Category: Qi D]]
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[[Category: Zhu, J]]
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[[Category: Wriggers W]]
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[[Category: Contractile protein]]
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[[Category: Zhu J]]
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[[Category: Cryo-em]]
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[[Category: Flexible docking]]
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[[Category: Inter- and intramolecular interaction]]
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[[Category: Myosin interacting-heads motif]]
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[[Category: Myosin regulation]]
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[[Category: Single particle reconstruction]]
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[[Category: Striated muscle]]
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[[Category: Super-relaxation]]
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[[Category: Tarantula]]
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[[Category: Thick filament]]
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Current revision

TWO HEAVY MEROMYOSIN INTERACTING-HEADS MOTIFS FLEXIBLE DOCKED INTO TARANTULA THICK FILAMENT 3D-MAP ALLOWS IN DEPTH STUDY OF INTRA- AND INTERMOLECULAR INTERACTIONS

3jbh, resolution 20.00Å

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