5f2z
From Proteopedia
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==Crystal structure of membrane associated PatA from Mycobacterium smegmatis in complex with palmitate - P21 space group== | ==Crystal structure of membrane associated PatA from Mycobacterium smegmatis in complex with palmitate - P21 space group== | ||
| - | <StructureSection load='5f2z' size='340' side='right' caption='[[5f2z]], [[Resolution|resolution]] 2.90Å' scene=''> | + | <StructureSection load='5f2z' size='340' side='right'caption='[[5f2z]], [[Resolution|resolution]] 2.90Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5f2z]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F2Z OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[5f2z]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F2Z FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f2z OCA], [https://pdbe.org/5f2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f2z RCSB], [https://www.ebi.ac.uk/pdbsum/5f2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f2z ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/ACYLT_MYCS2 ACYLT_MYCS2] Catalyzes the acylation to the position 6 of the alpha-1,2-linked mannose residue of the phosphatidyl-myo-inositol dimannoside (PIM2) or monomannoside (PIM1).<ref>PMID:12851411</ref> |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The biosynthesis of phospholipids and glycolipids are critical pathways for virtually all cell membranes. PatA is an essential membrane associated acyltransferase involved in the biosynthesis of mycobacterial phosphatidyl-myo-inositol mannosides (PIMs). The enzyme transfers a palmitoyl moiety from palmitoyl-CoA to the 6-position of the mannose ring linked to 2-position of inositol in PIM1/PIM2. We report here the crystal structures of PatA from Mycobacterium smegmatis in the presence of its naturally occurring acyl donor palmitate and a nonhydrolyzable palmitoyl-CoA analog. The structures reveal an alpha/beta architecture, with the acyl chain deeply buried into a hydrophobic pocket that runs perpendicular to a long groove where the active site is located. Enzyme catalysis is mediated by an unprecedented charge relay system, which markedly diverges from the canonical HX4D motif. Our studies establish the mechanistic basis of substrate/membrane recognition and catalysis for an important family of acyltransferases, providing exciting possibilities for inhibitor design. | ||
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| + | Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA.,Albesa-Jove D, Svetlikova Z, Tersa M, Sancho-Vaello E, Carreras-Gonzalez A, Bonnet P, Arrasate P, Eguskiza A, Angala SK, Cifuente JO, Kordulakova J, Jackson M, Mikusova K, Guerin ME Nat Commun. 2016 Mar 11;7:10906. doi: 10.1038/ncomms10906. PMID:26965057<ref>PMID:26965057</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5f2z" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Albesa-Jove | + | [[Category: Large Structures]] |
| - | [[Category: Carreras-Gonzalez | + | [[Category: Mycolicibacterium smegmatis MC2 155]] |
| - | [[Category: Cifuente | + | [[Category: Albesa-Jove D]] |
| - | [[Category: Guerin | + | [[Category: Carreras-Gonzalez A]] |
| - | [[Category: Mikusova | + | [[Category: Cifuente JO]] |
| - | [[Category: Sancho-Vaello | + | [[Category: Guerin ME]] |
| - | [[Category: Svetlikova | + | [[Category: Mikusova K]] |
| - | [[Category: Tersa | + | [[Category: Sancho-Vaello E]] |
| - | + | [[Category: Svetlikova Z]] | |
| - | + | [[Category: Tersa M]] | |
| - | + | ||
Current revision
Crystal structure of membrane associated PatA from Mycobacterium smegmatis in complex with palmitate - P21 space group
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