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| - | [[Image:1gwm.jpg|left|200px]] | |
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| - | {{Structure
| + | ==Carbohydrate binding module family29 complexed with glucohexaose== |
| - | |PDB= 1gwm |SIZE=350|CAPTION= <scene name='initialview01'>1gwm</scene>, resolution 1.15Å
| + | <StructureSection load='1gwm' size='340' side='right'caption='[[1gwm]], [[Resolution|resolution]] 1.15Å' scene=''> |
| - | |SITE= <scene name='pdbsite=AC1:Edo+Binding+Site+For+Chain+A'>AC1</scene>
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>
| + | <table><tr><td colspan='2'>[[1gwm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Piromyces_sp._'equi' Piromyces sp. 'equi']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GWM FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.15Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gwm OCA], [https://pdbe.org/1gwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gwm RCSB], [https://www.ebi.ac.uk/pdbsum/1gwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gwm ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gwm OCA], [http://www.ebi.ac.uk/pdbsum/1gwm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gwm RCSB]</span>
| + | == Function == |
| - | }}
| + | [https://www.uniprot.org/uniprot/Q9C171_PIREQ Q9C171_PIREQ] |
| - | | + | __TOC__ |
| - | '''CARBOHYDRATE BINDING MODULE FAMILY29 COMPLEXED WITH GLUCOHEXAOSE'''
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | | + | [[Category: Piromyces sp. 'equi']] |
| - | ==Overview==
| + | [[Category: Charnock SJ]] |
| - | Carbohydrate-protein recognition is central to many biological processes. Enzymes that act on polysaccharide substrates frequently contain noncatalytic domains, "carbohydrate-binding modules" (CBMs), that target the enzyme to the appropriate substrate. CBMs that recognize specific plant structural polysaccharides are often able to accommodate both the variable backbone and the side-chain decorations of heterogeneous ligands. "CBM29" modules, derived from a noncatalytic component of the Piromyces equi cellulase/hemicellulase complex, provide an example of this selective yet flexible recognition. They discriminate strongly against some polysaccharides while remaining relatively promiscuous toward both beta-1,4-linked manno- and cello-oligosaccharides. This feature may reflect preferential, but flexible, targeting toward glucomannans in the plant cell wall. The three-dimensional structure of CBM29-2 and its complexes with cello- and mannohexaose reveal a beta-jelly-roll topology, with an extended binding groove on the concave surface. The orientation of the aromatic residues complements the conformation of the target sugar polymer while accommodation of both manno- and gluco-configured oligo- and polysaccharides is conferred by virtue of the plasticity of the direct interactions from their axial and equatorial 2-hydroxyls, respectively. Such flexible ligand recognition targets the anaerobic fungal complex to a range of different components in the plant cell wall and thus plays a pivotal role in the highly efficient degradation of this composite structure by the microbial eukaryote.
| + | [[Category: Davies GJ]] |
| - | | + | [[Category: Nurizzo D]] |
| - | ==About this Structure==
| + | |
| - | 1GWM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Piromyces_equi Piromyces equi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GWM OCA].
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| - | | + | |
| - | ==Reference== | + | |
| - | Promiscuity in ligand-binding: The three-dimensional structure of a Piromyces carbohydrate-binding module, CBM29-2, in complex with cello- and mannohexaose., Charnock SJ, Bolam DN, Nurizzo D, Szabo L, McKie VA, Gilbert HJ, Davies GJ, Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14077-82. Epub 2002 Oct 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12391332 12391332]
| + | |
| - | [[Category: Piromyces equi]] | + | |
| - | [[Category: Single protein]] | + | |
| - | [[Category: Charnock, S J.]] | + | |
| - | [[Category: Davies, G J.]] | + | |
| - | [[Category: Nurizzo, D.]] | + | |
| - | [[Category: carbohydrate binding domain]]
| + | |
| - | [[Category: cellohexaose]]
| + | |
| - | [[Category: cellulosome]]
| + | |
| - | [[Category: glucomannan]]
| + | |
| - | [[Category: mannohexaose]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:52:52 2008''
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