This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
User:John S. de Banzie/Sandbox 4
From Proteopedia
< User:John S. de Banzie(Difference between revisions)
m |
m |
||
| (2 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
==Yeast U4/U6.U5 tri-snRNP== | ==Yeast U4/U6.U5 tri-snRNP== | ||
<StructureSection load='5gan' size='640' side='right' caption='U4/U6.U5 snRNP' [[5gan]]' scene='72/726441/Firstscene/2'> | <StructureSection load='5gan' size='640' side='right' caption='U4/U6.U5 snRNP' [[5gan]]' scene='72/726441/Firstscene/2'> | ||
| - | The <scene name='72/726441/Firstscene/2'>initial view</scene> shows proteins in white and the three snRNAs in colour (U4, U5, and U6). | + | The <scene name='72/726441/Firstscene/2'>initial view</scene> shows proteins in white and the three snRNAs in colour (U4: orange, U5: red, and U6: purple). |
| - | Focusing <scene name='72/726441/Rnascene/1'>on the RNAs</scene> reveals extensive base pairing between the U4 and U6 snRNAs. | + | Focusing <scene name='72/726441/Rnascene/1'>on the RNAs</scene> reveals secondary structure and extensive base pairing between the U4 and U6 snRNAs. |
| - | The U2 snRNP <scene name='72/726441/Dockscene/1'> | + | The spliceosome is assembled from the U4/U6.U5 tri-snRNP, the U1 snRNP, and the U2 snRNP. The U2 snRNP interacts with the region highlighted in blue <scene name='72/726441/Dockscene/1'>in this view</scene>. The U1 snRNP interacts with the U2 snRNP. The initial assembly is inactive; extensive conformational changes (including unwinding of the U4/U6 base pairing) are required for activation of the spliceosome. |
For the source article see Nguyen et al, 2016<ref>DOI 10.1038/nature16940</ref>. | For the source article see Nguyen et al, 2016<ref>DOI 10.1038/nature16940</ref>. | ||
| - | |||
| - | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
</StructureSection> | </StructureSection> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Current revision
Yeast U4/U6.U5 tri-snRNP
| |||||||||||
References
- ↑ Nguyen TH, Galej WP, Bai XC, Oubridge C, Newman AJ, Scheres SH, Nagai K. Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 A resolution. Nature. 2016 Feb 18;530(7590):298-302. doi: 10.1038/nature16940. Epub 2016 Feb 1. PMID:26829225 doi:http://dx.doi.org/10.1038/nature16940
