1gz9

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[[Image:1gz9.gif|left|200px]]
 
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{{Structure
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==High-Resolution Crystal Structure of Erythrina cristagalli Lectin in Complex with 2'-alpha-L-Fucosyllactose==
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|PDB= 1gz9 |SIZE=350|CAPTION= <scene name='initialview01'>1gz9</scene>, resolution 1.70&Aring;
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<StructureSection load='1gz9' size='340' side='right'caption='[[1gz9]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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|SITE= <scene name='pdbsite=MNA:Fuc+Binding+Site+For+Chain+A'>MNA</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=LAT:LACTOSE'>LAT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
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<table><tr><td colspan='2'>[[1gz9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Erythrina_crista-galli Erythrina crista-galli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GZ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GZ9 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PRD_900070:2-fucosyllactose'>PRD_900070</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gz9 OCA], [https://pdbe.org/1gz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gz9 RCSB], [https://www.ebi.ac.uk/pdbsum/1gz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gz9 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gz9 OCA], [http://www.ebi.ac.uk/pdbsum/1gz9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gz9 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/LEC_ERYCG LEC_ERYCG]
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== Evolutionary Conservation ==
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'''HIGH-RESOLUTION CRYSTAL STRUCTURE OF ERYTHRINA CRISTAGALLI LECTIN IN COMPLEX WITH 2'-ALPHA-L-FUCOSYLLACTOSE'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gz/1gz9_consurf.spt"</scriptWhenChecked>
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The primary sequence of Erythrina cristagalli lectin (ECL) was mapped by mass spectrometry, and the crystal structures of the lectin in complex with lactose and 2'-alpha-L-fucosyllactose were determined at 1.6A and 1.7A resolution, respectively. The two complexes were compared with the crystal structure of the closely related Erythrina corallodendron lectin (ECorL) in complex with lactose, with the crystal structure of the Ulex europaeus lectin II in complex with 2'-alpha-L-fucosyllactose, and with two modeled complexes of ECorL with 2'-alpha-L-fucosyl-N-acetyllactosamine. The molecular models are very similar to the crystal structure of ECL in complex with 2'-alpha-L-fucosyllactose with respect to the overall mode of binding, with the L-fucose fitting snugly into the cavity surrounded by Tyr106, Tyr108, Trp135 and Pro134 adjoining the primary combining site of the lectin. Marked differences were however noted between the models and the experimental structure in the network of hydrogen bonds and hydrophobic interactions holding the L-fucose in the combining site of the lectin, pointing to limitations of the modeling approach. In addition to the structural characterization of the ECL complexes, an effort was undertaken to correlate the structural data with thermodynamic data obtained from microcalorimetry, revealing the importance of the water network in the lectin combining site for carbohydrate binding.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1GZ9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GZ9 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gz9 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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High-resolution crystal structures of Erythrina cristagalli lectin in complex with lactose and 2'-alpha-L-fucosyllactose and correlation with thermodynamic binding data., Svensson C, Teneberg S, Nilsson CL, Kjellberg A, Schwarz FP, Sharon N, Krengel U, J Mol Biol. 2002 Aug 2;321(1):69-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12139934 12139934]
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</StructureSection>
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[[Category: Eukaryota]]
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[[Category: Erythrina crista-galli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kjellberg, A.]]
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[[Category: Kjellberg A]]
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[[Category: Krengel, U.]]
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[[Category: Krengel U]]
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[[Category: Nilsson, C L.]]
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[[Category: Nilsson CL]]
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[[Category: Schwarz, F P.]]
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[[Category: Schwarz FP]]
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[[Category: Sharon, N.]]
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[[Category: Sharon N]]
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[[Category: Svensson, C.]]
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[[Category: Svensson C]]
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[[Category: Teneberg, S.]]
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[[Category: Teneberg S]]
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[[Category: carbohydrate]]
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[[Category: crystal structure]]
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[[Category: fucose]]
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[[Category: fucosyllactose protein-carbohydrate interaction]]
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[[Category: glycobiology]]
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[[Category: glycoprotein]]
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[[Category: lactose]]
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[[Category: legume lectin]]
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[[Category: protein]]
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[[Category: saccharide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:54:28 2008''
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Current revision

High-Resolution Crystal Structure of Erythrina cristagalli Lectin in Complex with 2'-alpha-L-Fucosyllactose

PDB ID 1gz9

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