Isopenicillin N synthase
From Proteopedia
(Difference between revisions)
| (6 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | + | <StructureSection load='1blz' size='350' side='right' scene='52/525170/Cv/1' caption='Isopenicilln N synthase complex with ACV and NO, [[1blz]]'> | |
| + | |||
== Function == | == Function == | ||
'''Isopenicillin N synthase''' (IPNS) is an iron-dependent enzyme which catalyzes the formation of isopenicillin N (IPN) from the tripeptide aminoadipoyl-cysteine-valine (ACV). IPNS participates in the biosynthesis of penicillin and cephalosporin antibiotics. The active site of IPNS contains an Fe atom. The reaction involves the reduction of O2 molecule to H2O<ref>PMID:10537113</ref>. | '''Isopenicillin N synthase''' (IPNS) is an iron-dependent enzyme which catalyzes the formation of isopenicillin N (IPN) from the tripeptide aminoadipoyl-cysteine-valine (ACV). IPNS participates in the biosynthesis of penicillin and cephalosporin antibiotics. The active site of IPNS contains an Fe atom. The reaction involves the reduction of O2 molecule to H2O<ref>PMID:10537113</ref>. | ||
== Structural highlights == | == Structural highlights == | ||
| - | IPNS active site contains Fe+2 and the substrate ACV. | + | IPNS <scene name='52/525170/Cv/7'>active site contains Fe+2 and the substrate ACV</scene>. The <scene name='52/525170/Cv/8'>Fe+2 ion is hexacoordinated to 3 IPNS side chains, one water molecule, the ACV thiolate moiety and NO</scene><ref>PMID:9194566</ref>. Water molecules shown as red spheres. |
| - | + | </StructureSection> | |
==3D structures of isopenicillin N synthase== | ==3D structures of isopenicillin N synthase== | ||
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| Line 13: | Line 14: | ||
**[[1bk0]] - AnIPNS + ACV – ''Aspergillus nidulans''<br /> | **[[1bk0]] - AnIPNS + ACV – ''Aspergillus nidulans''<br /> | ||
| - | **[[2bjs]] - AnIPNS (mutant) + ACV<br /> | + | **[[2bjs]], [[7zoe]] - AnIPNS (mutant) + ACV<br /> |
| - | **[[1qiq]], [[1qjf]], [[1hb1]], [[1hb2]], [[1hb3]], [[1hb4]], [[1odm]], [[1odn]], [[1oc1]], [[1uzw]], [[1w03]], [[1w05]], [[2bu9]], [[1w3v]], [[1w3x]], [[2ivi]], [[2ivj]], [[2vau]], [[2vbb]], [[2vbd]], [[2vbp]], [[2vcm]], [[2ve1]], [[2wo7]], [[2y6f]], [[2y60 | + | **[[1qiq]], [[1qjf]], [[1hb1]], [[1hb2]], [[1hb3]], [[1hb4]], [[1odm]], [[1odn]], [[1oc1]], [[1uzw]], [[1w03]], [[1w05]], [[2bu9]], [[1w3v]], [[1w3x]], [[2ivi]], [[2ivj]], [[2vau]], [[2vbb]], [[2vbd]], [[2vbp]], [[2vcm]], [[2ve1]], [[2wo7]], [[2y6f]], [[2y60]], [[4bb3]] - AnIPNS + ACV derivative<br /> |
**[[2jb4]] - AnIPNS + cyclobutanone analog<br /> | **[[2jb4]] - AnIPNS + cyclobutanone analog<br /> | ||
| + | **[[6y0p]], [[6zao]], [[6zaq]] - AnIPNS + IPN<br /> | ||
**[[1qje]] - AnIPNS + IPN + ACV<br /> | **[[1qje]] - AnIPNS + IPN + ACV<br /> | ||
| - | **[[1blz]] - AnIPNS + NO + ACV<br /> | + | **[[6y0o]], [[6zae]], [[6zaf]], [[6zag]], [[6zah]], [[6zam]] - AnIPNS + ACV<br /> |
| + | **[[6zai]], [[6zaj]], [[6zal]], [[6zap]] - AnIPNS + O2 + ACV<br /> | ||
| + | **[[7poy]] - AnIPNS (mutant) + NO + ACV<br /> | ||
| + | **[[7psw]] - AnIPNS (mutant) + Fe + ACV<br /> | ||
| + | **[[1blz]], [[6zan]] - AnIPNS + NO + ACV<br /> | ||
**[[1obn]], [[1w04]], [[1w06]] - AnIPNS + hydroxylamine + ACV derivative<br /> | **[[1obn]], [[1w04]], [[1w06]] - AnIPNS + hydroxylamine + ACV derivative<br /> | ||
**[[3zku]], [[3zky]], [[3zoi]], [[4bb3]] - IPNS + ACV derivative – ''Emericella nidulans''<br /> | **[[3zku]], [[3zky]], [[3zoi]], [[4bb3]] - IPNS + ACV derivative – ''Emericella nidulans''<br /> | ||
| - | * | + | *Other metal containing IPNS |
| - | **[[1ips]] – AnIPNS | + | **[[1ips]] – AnIPNS + Mn<br /> |
| + | **[[6zw8]] - AnIPNS + ACV + Cd<br /> | ||
}} | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category: Topic Page]] | [[Category: Topic Page]] | ||
Current revision
| |||||||||||
3D structures of isopenicillin N synthase
Updated on 12-June-2023
References
- ↑ Burzlaff NI, Rutledge PJ, Clifton IJ, Hensgens CM, Pickford M, Adlington RM, Roach PL, Baldwin JE. The reaction cycle of isopenicillin N synthase observed by X-ray diffraction. Nature. 1999 Oct 14;401(6754):721-4. PMID:10537113 doi:10.1038/44400
- ↑ Roach PL, Clifton IJ, Hensgens CM, Shibata N, Schofield CJ, Hajdu J, Baldwin JE. Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation. Nature. 1997 Jun 19;387(6635):827-30. PMID:9194566 doi:http://dx.doi.org/10.1038/42990
