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Inorganic pyrophosphatase

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<StructureSection load='2bqy' size='450' side='right' scene='' caption='Inorganic pyrophosphatase complex with pyrophosphate, [[2bqy]]'>
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<StructureSection load='2bqy' size='350' side='right' scene='48/488499/Cv/1' caption='Inorganic pyrophosphatase complex with pyrophosphate, [[2bqy]]'>
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__TOC__
== Function ==
== Function ==
'''Inorganic pyrophosphatase''' (PPase) catalyzes the breakdown of pyrophosphate (PPi) to two molecules of phosphate (PO4). The reaction releases energy and is irreversible hence it is coupled to biochemical reactions which need to be driven to completion. PPase plays a role in lipid metabolism, calcium absorption, bone formation and DNA synthesis. PPase requires a divalent metal ion like Mg+2 for higher activity. PPi is formed in many biosynthetic reactions which require ATP<ref>PMID:5342521</ref>.
'''Inorganic pyrophosphatase''' (PPase) catalyzes the breakdown of pyrophosphate (PPi) to two molecules of phosphate (PO4). The reaction releases energy and is irreversible hence it is coupled to biochemical reactions which need to be driven to completion. PPase plays a role in lipid metabolism, calcium absorption, bone formation and DNA synthesis. PPase requires a divalent metal ion like Mg+2 for higher activity. PPi is formed in many biosynthetic reactions which require ATP<ref>PMID:5342521</ref>.
== Structural highlights ==
== Structural highlights ==
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PPase binding pocket has negative electrostatic potential and is composed of 3 lysine residues, a tyrosine and several water molecules<ref>PMID:16988955</ref>.
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The biological assembly of Inorganic pyrophosphatase ''Helicobacter pylori'' is <scene name='48/488499/Cv/6'>homohexamer</scene>. PPase <scene name='48/488499/Cv/7'>binding pocket has electrostatic potential and is composed of 3 lysine residues, a tyrosine and several water molecules</scene><ref>PMID:16988955</ref>. {{Template:ColorKey_Charge_Anionic}} / {{Template:ColorKey_Charge_Cationic}}. Water molecules shown as red spheres.
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</StructureSection>
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==3D structures of inorganic pyrophosphatase==
==3D structures of inorganic pyrophosphatase==
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[[Inorganic pyrophosphatase 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Pyrophosphatase
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**[[1pyp]] – yPPase – yeast<br />
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**[[1igp]], [[1faj]], [[2eip]], [[1jfd]], [[4um4]] – EcPPase – ''Escherichia coli''<br />
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**[[1mjw]], [[1mjx]], [[1mjy]], [[1mjz]] - EcPPase (mutant)<br />
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**[[1b78]] - MjPPase – ''Methanocaldococcus jannaschii''<br />
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**[[1ude]] – PPase – ''Pyrococcus horikoshii''<br />
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**[[1twl]] – PPase – ''Pyrococcus furiosus''<br />
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**[[1wpm]] - BsPPase – ''Bacillus subtilis''<br />
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**[[1sxv]] - MtPPase – ''Mycobacterium tuberculosis''<br />
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**[[4ecp]] - PPase – ''Mycobacterium leprae''<br />
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**[[1ygz]], [[2bqx]] - HpPPase - ''Helicobacter pylori''<br />
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**[[3d53]], [[3emj]] - PPase – ''Rickettsia prowazekii''<br />
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**[[3d63]], [[3eiz]] - BpPPase – ''Burkholderia pseudomallei''<br />
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**[[3lo0]] - PPase – ''Ehrlichia chafeensis''<br />
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**[[3qu5]], [[3qub]], [[3quc]] - BtPPase (mutant) – ''Bacterioides thetaiotaomicron''<br />
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**[[3sw5]] – PPase – ''Bartonella henselae''<br />
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**[[2prd]] – PPase – ''Thermus thermophilus''<br />
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**[[3r6e]] – TtPPase – ''Thermococcus thioreducens''<br />
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**[[3q3l]] – TtPPase – neutron diffraction<br />
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**[[4lug]] - PPase – ''Arabidopsis thaliana''<br />
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*Pyrophosphatase binary complex
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**[[1ino]] – EcPPase + Mn<br />
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**[[1ipw]], [[1obw]] - EcPPase + Mg<br />
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**[[1i40]] - EcPPase + Ca<br />
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**[[1wgi]] - yPPase + Mn<br />
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**[[1huk]] - yPPase + Mg<br />
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**[[1huj]], [[2ik1]] - yPPase (mutant) + Mg<br />
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**[[1qez]] - PPase + Mg – ''Sulfolobus acidocaldarius''<br />
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**[[3r5u]] – TtPPase + Mn <br />
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**[[3r5v]] – TtPPase + Ca<br />
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**[[2mjp]] - MjPPase + AMPPNP<br />
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**[[2eb0]] - MjPPase + Mn <br />
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**[[1k20]] - SgPPase + Mn – ''Streptococcus gordonii''<br />
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**[[1wpp]] - SgPPase + Zn<br />
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**[[1k23]] - BsPPase + Mn<br />
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**[[1wpn]] - BsPPase N terminal + Mn<br />
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**[[2bqy]] - HpPPase + PPi<br />
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**[[2uxs]] - MtPPase + PO4<br />
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**[[3ej0]] - BpPPase + pyridine derivative<br />
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**[[3ej2]] - BpPPase + pyrazole derivative<br />
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**[[3fq3]] - PPase + PO4 – ''Brucella melitensis''<br />
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**[[3ld3]] - PPase + PO4 – ''Anaplasma phagocytophilum''<br />
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**[[3obc]] - PPase + Zn – ''Archaeoglobus fulgidus''<br />
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**[[3quq]], [[3qu2]] - BtPPase + Mg<br />
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**[[3qut]], [[3qu4]] - BtPPase (mutant) + Mg<br />
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**[[4xel]] – PPase + Mg – ''Pseudomonas aeruginosa''<br />
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*Pyrophosphatase ternary complex
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**[[1ypp]], [[1wgj]], [[1e6a]], [[1e9g]] – yPPase + PO4 + Mn<br />
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**[[1m38]] - yPPase + PO4 + Co<br />
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**[[2ihp]] - yPPase + PO4 + Mg<br />
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**[[117e]], [[8prk]] - yPPase (mutant) + PO4 + Mn<br />
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**[[2ik0]], [[2ik2]], [[2ik4]], [[2ik6]], [[2ik7]], [[2ik9]] - yPPase (mutant) + PO4 + Mg<br />
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**[[1wcf]] - MtPPase + PO4 + K<br />
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**[[1i6t]] - EcPPase + PPi + Ca <br />
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**[[2au9]] - EcPPase + PPi + Mn<br />
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**[[2auu]] - EcPPase + PPi + Mg<br />
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**[[2au7]] – EcPPase (mutant) + PO4 + Mn<br />
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**[[2au8]] - EcPPase + PO4 + Mn<br />
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**[[2haw]] - BsPPase + imidodiphosphoric acid + Mg<br />
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**[[3qyp]], [[3qu7]] - BtPPase (mutant) + PO4 + Ca<br />
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**[[3qu9]] - BtPPase (mutant) + tartarate + Mg<br />
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**[[3qx7]] - BtPPase + PO4 + Mg<br />
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**[[3tr4]] - PPase + Mg + Mn – ''Coxiella burnetii''<br />
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**[[3q46]] - TtPPase + PO4 + Mg<br />
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**[[3q5v]] - TtPPase + SO4 + Mg<br />
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**[[3q4w]], [[4q9m]] - TtPPase + PPi + Ca<br />
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*Pyrophosphatase quaternary complex
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**[[2au6]] - EcPPase + PPi + PO4 + Mn<br />
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**[[2iw4]] - BsPPase + imidodiphosphoric acid + Fe + Mg + Mn<br />
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**[[2enx]] - PPase + imidodiphosphoric acid + Mg + Mn – ''Streptococcus agalactiae''<br />
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**[[3eiy]] - BpPPase + PPi + Na + K<br />
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**[[3gvf]] - BpPPase + PO4 + Na + K<br />
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**[[3qxg]] - BtPPase + PO4 + Mg + Ca
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*NADH pyrophosphatase
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**[[1vk6]], [[2gb5]] – EcPPase + Zn<br />
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*Proton pyrophosphatase
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**[[4a01]] – PPase + imidodiphosphoric acid + Mg + K – ''Vigna radiata''
 
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}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Inorganic pyrophosphatase complex with pyrophosphate, 2bqy

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References

  1. ↑ Harold FM. Inorganic polyphosphates in biology: structure, metabolism, and function. Bacteriol Rev. 1966 Dec;30(4):772-94. PMID:5342521
  2. ↑ Chao TC, Huang H, Tsai JY, Huang CY, Sun YJ. Kinetic and structural properties of inorganic pyrophosphatase from the pathogenic bacterium Helicobacter pylori. Proteins. 2006 Nov 15;65(3):670-80. PMID:16988955 doi:10.1002/prot.21093

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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