Ire1
From Proteopedia
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<StructureSection load='3lj0' size='400' side='right' caption='Structure of yeast Ire1 cytoplasmic domain dimer complex with quercetin, ADP, Ca+2 and Sr+2 ions (PDB entry [[3lj0]])' scene='51/516469/Cv/1'> | <StructureSection load='3lj0' size='400' side='right' caption='Structure of yeast Ire1 cytoplasmic domain dimer complex with quercetin, ADP, Ca+2 and Sr+2 ions (PDB entry [[3lj0]])' scene='51/516469/Cv/1'> | ||
== Function == | == Function == | ||
| - | '''Ire1''' | + | '''Ire1''' or '''serine/threonine protein kinase/endoribonuclease Ire1'''. It is important in altering gene expression as a response to endoplasmic reticulum-based stress signals<ref>PMID:11034898</ref>. The endoribonuclase domain of Ire1 is a transcriptional activator which triggers growth arrest and apoptosis. The kinase domain of Ire1 is required for activation of the endoribonuclase domain. Ire1 senses unfolded proteins causing its auto-activation. |
== Structural highlights == | == Structural highlights == | ||
| - | Yeast Ire1 structure contains 3 phosphorylated residues: <scene name='51/516469/Cv/ | + | Yeast Ire1 structure contains 3 phosphorylated residues: <scene name='51/516469/Cv/9'>2 Ser and a Thr</scene>. Ire1 shows a different <scene name='51/516469/Cv/10'>binding site for ADP</scene> and for quercetin. Quercetin is a powerful activator of Ire1. <scene name='51/516469/Cv/11'>Ire1 binds 2 molecules of quercetin at its dimer interface</scene><ref>PMID:23880584</ref>. |
==3D structures of Ire1== | ==3D structures of Ire1== | ||
| + | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | [[2hz6]] – hIre1 | + | [[2hz6]], [[6shc]] – hIre1 luminal domain 24-419 (mutant) – human<br /> |
| + | [[4z7g]], [[5hgi]], [[6w3b]], [[6w3c]] – hIre1 cytoplasmic domain 547-977<br /> | ||
| + | [[3p23]], [[4yzd]] - hIre1 cytoplasmic domain + ADP<br /> | ||
| + | [[4u6r]], [[4yz9]], [[4z7h]], [[6urc]], [[6hv0]], [[6hx1]], [[6w39]], [[6w3a]], [[6w3e]], [[6w3k]], [[6xdb]], [[6xdd]], [[6xdf]], [[7bmk]] – hIre1 cytoplasmic domain + inhibitor <br /> | ||
| + | [[4yzc]] – hIre1 cytoplasmic domain + satuorosporine <br /> | ||
| + | [[4pl3]], [[4pl4]], [[4pl5]] – mIre1 cytoplasmic domain (mutant) + inhibitor – mouse<br /> | ||
[[2rio]], [[3fbv]], [[3sdm]] – yIre1 cytoplasmic domain – yeast<br /> | [[2rio]], [[3fbv]], [[3sdm]] – yIre1 cytoplasmic domain – yeast<br /> | ||
[[3sdj]] - yIre1 cytoplasmic domain (mutant) <br /> | [[3sdj]] - yIre1 cytoplasmic domain (mutant) <br /> | ||
| - | [[ | + | [[2be1]] – yIre1 lumenal domain + peptide<br /> |
[[3lj0]] - yIre1 cytoplasmic domain + quercetin + ADP<br /> | [[3lj0]] - yIre1 cytoplasmic domain + quercetin + ADP<br /> | ||
[[3lj1]], [[3lj2]] - yIre1 cytoplasmic domain + inhibitor<br /> | [[3lj1]], [[3lj2]] - yIre1 cytoplasmic domain + inhibitor<br /> | ||
| - | + | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
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