Isopenicillin N synthase
From Proteopedia
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| - | <StructureSection load='1blz' size=' | + | <StructureSection load='1blz' size='350' side='right' scene='52/525170/Cv/1' caption='Isopenicilln N synthase complex with ACV and NO, [[1blz]]'> |
== Function == | == Function == | ||
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== Structural highlights == | == Structural highlights == | ||
| - | IPNS <scene name='52/525170/Cv/ | + | IPNS <scene name='52/525170/Cv/7'>active site contains Fe+2 and the substrate ACV</scene>. The <scene name='52/525170/Cv/8'>Fe+2 ion is hexacoordinated to 3 IPNS side chains, one water molecule, the ACV thiolate moiety and NO</scene><ref>PMID:9194566</ref>. Water molecules shown as red spheres. |
</StructureSection> | </StructureSection> | ||
==3D structures of isopenicillin N synthase== | ==3D structures of isopenicillin N synthase== | ||
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**[[1bk0]] - AnIPNS + ACV – ''Aspergillus nidulans''<br /> | **[[1bk0]] - AnIPNS + ACV – ''Aspergillus nidulans''<br /> | ||
| - | **[[2bjs]] - AnIPNS (mutant) + ACV<br /> | + | **[[2bjs]], [[7zoe]] - AnIPNS (mutant) + ACV<br /> |
| - | **[[1qiq]], [[1qjf]], [[1hb1]], [[1hb2]], [[1hb3]], [[1hb4]], [[1odm]], [[1odn]], [[1oc1]], [[1uzw]], [[1w03]], [[1w05]], [[2bu9]], [[1w3v]], [[1w3x]], [[2ivi]], [[2ivj]], [[2vau]], [[2vbb]], [[2vbd]], [[2vbp]], [[2vcm]], [[2ve1]], [[2wo7]], [[2y6f]], [[2y60 | + | **[[1qiq]], [[1qjf]], [[1hb1]], [[1hb2]], [[1hb3]], [[1hb4]], [[1odm]], [[1odn]], [[1oc1]], [[1uzw]], [[1w03]], [[1w05]], [[2bu9]], [[1w3v]], [[1w3x]], [[2ivi]], [[2ivj]], [[2vau]], [[2vbb]], [[2vbd]], [[2vbp]], [[2vcm]], [[2ve1]], [[2wo7]], [[2y6f]], [[2y60]], [[4bb3]] - AnIPNS + ACV derivative<br /> |
**[[2jb4]] - AnIPNS + cyclobutanone analog<br /> | **[[2jb4]] - AnIPNS + cyclobutanone analog<br /> | ||
| + | **[[6y0p]], [[6zao]], [[6zaq]] - AnIPNS + IPN<br /> | ||
**[[1qje]] - AnIPNS + IPN + ACV<br /> | **[[1qje]] - AnIPNS + IPN + ACV<br /> | ||
| - | **[[1blz]] - AnIPNS + NO + ACV<br /> | + | **[[6y0o]], [[6zae]], [[6zaf]], [[6zag]], [[6zah]], [[6zam]] - AnIPNS + ACV<br /> |
| + | **[[6zai]], [[6zaj]], [[6zal]], [[6zap]] - AnIPNS + O2 + ACV<br /> | ||
| + | **[[7poy]] - AnIPNS (mutant) + NO + ACV<br /> | ||
| + | **[[7psw]] - AnIPNS (mutant) + Fe + ACV<br /> | ||
| + | **[[1blz]], [[6zan]] - AnIPNS + NO + ACV<br /> | ||
**[[1obn]], [[1w04]], [[1w06]] - AnIPNS + hydroxylamine + ACV derivative<br /> | **[[1obn]], [[1w04]], [[1w06]] - AnIPNS + hydroxylamine + ACV derivative<br /> | ||
**[[3zku]], [[3zky]], [[3zoi]], [[4bb3]] - IPNS + ACV derivative – ''Emericella nidulans''<br /> | **[[3zku]], [[3zky]], [[3zoi]], [[4bb3]] - IPNS + ACV derivative – ''Emericella nidulans''<br /> | ||
| - | * | + | *Other metal containing IPNS |
| - | **[[1ips]] – AnIPNS | + | **[[1ips]] – AnIPNS + Mn<br /> |
| + | **[[6zw8]] - AnIPNS + ACV + Cd<br /> | ||
}} | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category: Topic Page]] | [[Category: Topic Page]] | ||
Current revision
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3D structures of isopenicillin N synthase
Updated on 12-June-2023
References
- ↑ Burzlaff NI, Rutledge PJ, Clifton IJ, Hensgens CM, Pickford M, Adlington RM, Roach PL, Baldwin JE. The reaction cycle of isopenicillin N synthase observed by X-ray diffraction. Nature. 1999 Oct 14;401(6754):721-4. PMID:10537113 doi:10.1038/44400
- ↑ Roach PL, Clifton IJ, Hensgens CM, Shibata N, Schofield CJ, Hajdu J, Baldwin JE. Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation. Nature. 1997 Jun 19;387(6635):827-30. PMID:9194566 doi:http://dx.doi.org/10.1038/42990
