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1hjk

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ALKALINE PHOSPHATASE MUTANT H331Q

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Retrieved from "http://52.214.119.220/wiki/index.php/1hjk"

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-[[Image:1hjk.gif|left|200px]]
- {{Structure
+==ALKALINE PHOSPHATASE MUTANT H331Q==
-|PDB= 1hjk |SIZE=350|CAPTION= <scene name='initialview01'>1hjk</scene>, resolution 2.3&Aring;
+<StructureSection load='1hjk' size='340' side='right'caption='[[1hjk]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-|SITE= <scene name='pdbsite=CTA:Catalytically+Active+Site+On+A+Subunit'>CTA</scene> and <scene name='pdbsite=CTB:Catalytically+Active+Site+On+B+Subunit'>CTB</scene>
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+<table><tr><td colspan='2'>[[1hjk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HJK FirstGlance]. <br>
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-|GENE= PHOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjk OCA], [https://pdbe.org/1hjk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hjk RCSB], [https://www.ebi.ac.uk/pdbsum/1hjk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hjk ProSAT]</span></td></tr>
-|RELATEDENTRY=
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjk OCA], [http://www.ebi.ac.uk/pdbsum/1hjk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hjk RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/PPB_ECOLI PPB_ECOLI]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/1hjk_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hjk ConSurf].
+<div style="clear:both"></div>
-'''ALKALINE PHOSPHATASE MUTANT H331Q'''
+==See Also==
+*[[Alkaline phosphatase 3D structures|Alkaline phosphatase 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-Using a mutant version of E. coli alkaline phosphatase, we succeeded in trapping and determining the structure of the phospho-enzyme intermediate. The X-ray structure also revealed the catalytic water molecule, bound to one of the active site zinc ions, positioned ideally for the apical attack necessary for the hydrolysis of the intermediate.
-==About this Structure==
-HJK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJK OCA].
-==Reference==
-Trapping and visualization of a covalent enzyme-phosphate intermediate., Murphy JE, Stec B, Ma L, Kantrowitz ER, Nat Struct Biol. 1997 Aug;4(8):618-22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9253408 9253408]
-[[Category: Alkaline phosphatase]]
 [[Category: Escherichia coli]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Kantrowitz, E R.]]
+[[Category: Kantrowitz ER]]
-[[Category: Ma, L.]]
+[[Category: Ma L]]
-[[Category: Murphy, J E.]]
+[[Category: Murphy JE]]
-[[Category: Stec, B.]]
+[[Category: Stec B]]
-[[Category: alcohol acceptor)]]
-[[Category: alkaline phosphatase]]
-[[Category: hydrolase]]
-[[Category: phosphoric monoester]]
-[[Category: transferase(phospho]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:06:08 2008''

1hjk

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ALKALINE PHOSPHATASE MUTANT H331Q

Structural highlights

Function

Evolutionary Conservation

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