1hjp

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[[Image:1hjp.jpg|left|200px]]
 
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{{Structure
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==HOLLIDAY JUNCTION BINDING PROTEIN RUVA FROM E. COLI==
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|PDB= 1hjp |SIZE=350|CAPTION= <scene name='initialview01'>1hjp</scene>, resolution 2.5&Aring;
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<StructureSection load='1hjp' size='340' side='right'caption='[[1hjp]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1hjp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HJP FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE= RUVA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hjp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjp OCA], [https://pdbe.org/1hjp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hjp RCSB], [https://www.ebi.ac.uk/pdbsum/1hjp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hjp ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hjp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjp OCA], [http://www.ebi.ac.uk/pdbsum/1hjp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hjp RCSB]</span>
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[https://www.uniprot.org/uniprot/RUVA_ECOLI RUVA_ECOLI] The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA.<ref>PMID:8433990</ref>
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/1hjp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hjp ConSurf].
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<div style="clear:both"></div>
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'''HOLLIDAY JUNCTION BINDING PROTEIN RUVA FROM E. COLI'''
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==See Also==
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*[[Helicase 3D structures|Helicase 3D structures]]
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== References ==
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==Overview==
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<references/>
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BACKGROUND: Homologous recombination is crucial for genetic diversity and repairing damaged chromosomes. In Escherichia coli cells, the RuvA, RuvB and RuvC proteins participate in the processing of an important intermediate, the Holliday junction. The RuvA-RuvB protein complex facilitates branch migration of the junction, depending on ATP hydrolysis. The atomic structure of RuvA should enable critical questions to be addressed about its specific interactions with the Holliday junction and the RuvB protein. RESULTS: The crystal structure of RuvA shows the tetrameric molecules with a fourfold axis at the center. Each subunit consists of three distinct domains, some of which contain important secondary structure elements for DNA binding. Together with the detailed structural information, the biochemical assays of various mutant RuvA proteins and domains, isolated by partial proteolysis, allowed us to define the functional roles of these domains in Holliday junction binding and the RuvB interaction. CONCLUSIONS: The RuvA molecule is formed by four identical subunits, each with three domains, I, II and III. The locations of the putative DNA-binding motifs define an interface between the DNA and the Holliday junction. Domain III is weakly attached to the core region, comprising domains I and II; the core domains can form a tetramer in the absence of domain III. Functional analyses of the mutant proteins and the partial digestion products, including Holliday junction binding and branch-migration assays, revealed that domain III and the preceding loop are crucial for RuvB binding and branch migration, although this region is not required for the junction-DNA binding.
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__TOC__
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</StructureSection>
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==About this Structure==
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1HJP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJP OCA].
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==Reference==
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Functional analyses of the domain structure in the Holliday junction binding protein RuvA., Nishino T, Ariyoshi M, Iwasaki H, Shinagawa H, Morikawa K, Structure. 1998 Jan 15;6(1):11-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9493263 9493263]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Ariyoshi, M.]]
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[[Category: Ariyoshi M]]
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[[Category: Iwasaki, H.]]
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[[Category: Iwasaki H]]
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[[Category: Morikawa, K.]]
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[[Category: Morikawa K]]
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[[Category: Nishino, T.]]
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[[Category: Nishino T]]
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[[Category: Shinagawa, H.]]
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[[Category: Shinagawa H]]
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[[Category: dna recombination]]
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[[Category: dna repair]]
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[[Category: holliday junction binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:06:11 2008''
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Current revision

HOLLIDAY JUNCTION BINDING PROTEIN RUVA FROM E. COLI

PDB ID 1hjp

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