5i69

From Proteopedia

(Difference between revisions)

Current revision

MBP-MamC magnetite-interaction component mutant-D70A

Structural highlights

5i69 is a 1 chain structure with sequence from Escherichia coli and Magnetospirillum magneticum AMB-1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.7Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MALE_ECOLI Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.MAMC_MAGSA Probably involved in magnetite crystal growth (Probable). The lumenal domain may bind the magnetite crystals, affecting crystal size and shape (Probable).^[1] ^[2] ^[3]

Publication Abstract from PubMed

Magnetotactic bacteria are Gram-negative bacteria that navigate along geomagnetic fields using the magnetosome, an organelle that consists of a membrane-enveloped magnetic nanoparticle. Magnetite formation and its properties are controlled by a specific set of proteins. MamC is a small magnetosome-membrane protein that is known to be active in iron biomineralization but its mechanism has yet to be clarified. Here, we studied the relationship between the MamC magnetite-interaction loop (MIL) structure and its magnetite interaction using an inert biomineralization protein-MamC chimera. Our determined structure shows an alpha-helical fold for MamC-MIL with highly charged surfaces. Additionally, the MamC-MIL induces the formation of larger magnetite crystals compared to protein-free and inert biomineralization protein control experiments. We suggest that the connection between the MamC-MIL structure and the protein's charged surfaces is crucial for magnetite binding and thus for the size control of the magnetite nanoparticles.

Structure-function studies of the magnetite-biomineralizing magnetosome-associated protein MamC.,Nudelman H, Valverde-Tercedor C, Kolusheva S, Perez Gonzalez T, Widdrat M, Grimberg N, Levi H, Nelkenbaum O, Davidov G, Faivre D, Jimenez-Lopez C, Zarivach R J Struct Biol. 2016 Jun;194(3):244-52. doi: 10.1016/j.jsb.2016.03.001. Epub 2016 , Mar 10. PMID:26970040^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Arakaki A, Yamagishi A, Fukuyo A, Tanaka M, Matsunaga T. Co-ordinated functions of Mms proteins define the surface structure of cubo-octahedral magnetite crystals in magnetotactic bacteria. Mol Microbiol. 2014 Aug;93(3):554-67. PMID:24961165 doi:10.1111/mmi.12683
↑ Nudelman H, Valverde-Tercedor C, Kolusheva S, Perez Gonzalez T, Widdrat M, Grimberg N, Levi H, Nelkenbaum O, Davidov G, Faivre D, Jimenez-Lopez C, Zarivach R. Structure-function studies of the magnetite-biomineralizing magnetosome-associated protein MamC. J Struct Biol. 2016 Jun;194(3):244-52. doi: 10.1016/j.jsb.2016.03.001. Epub 2016 , Mar 10. PMID:26970040 doi:http://dx.doi.org/10.1016/j.jsb.2016.03.001
↑ Nudelman H, Lee YZ, Hung YL, Kolusheva S, Upcher A, Chen YC, Chen JY, Sue SC, Zarivach R. Understanding the Biomineralization Role of Magnetite-Interacting Components (MICs) From Magnetotactic Bacteria. Front Microbiol. 2018 Oct 23;9:2480. PMID:30405554 doi:10.3389/fmicb.2018.02480
↑ Nudelman H, Valverde-Tercedor C, Kolusheva S, Perez Gonzalez T, Widdrat M, Grimberg N, Levi H, Nelkenbaum O, Davidov G, Faivre D, Jimenez-Lopez C, Zarivach R. Structure-function studies of the magnetite-biomineralizing magnetosome-associated protein MamC. J Struct Biol. 2016 Jun;194(3):244-52. doi: 10.1016/j.jsb.2016.03.001. Epub 2016 , Mar 10. PMID:26970040 doi:http://dx.doi.org/10.1016/j.jsb.2016.03.001

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5i69"

@@ Line 1: / Line 1: @@
 ==MBP-MamC magnetite-interaction component mutant-D70A==
-<StructureSection load='5i69' size='340' side='right' caption='[[5i69]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+<StructureSection load='5i69' size='340' side='right'caption='[[5i69]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5i69]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I69 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I69 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5i69]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Magnetospirillum_magneticum_AMB-1 Magnetospirillum magneticum AMB-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I69 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I69 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAL:MALTOSE'>MAL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i69 OCA], [http://pdbe.org/5i69 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i69 RCSB], [http://www.ebi.ac.uk/pdbsum/5i69 PDBsum]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i69 OCA], [https://pdbe.org/5i69 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i69 RCSB], [https://www.ebi.ac.uk/pdbsum/5i69 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i69 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.
+[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/MAMC_MAGSA MAMC_MAGSA] Probably involved in magnetite crystal growth (Probable). The lumenal domain may bind the magnetite crystals, affecting crystal size and shape (Probable).<ref>PMID:24961165</ref> <ref>PMID:26970040</ref> <ref>PMID:30405554</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Davidove, G]]
+[[Category: Escherichia coli]]
-[[Category: Faivre, D]]
+[[Category: Large Structures]]
-[[Category: Gonzalez, T P]]
+[[Category: Magnetospirillum magneticum AMB-1]]
-[[Category: Grimberg, N]]
+[[Category: Davidove G]]
-[[Category: Jimenez-Lopez, C]]
+[[Category: Faivre D]]
-[[Category: Kolusheva, S]]
+[[Category: Gonzalez TP]]
-[[Category: Levi, H]]
+[[Category: Grimberg N]]
-[[Category: Nelkenbaum, O]]
+[[Category: Jimenez-Lopez C]]
-[[Category: Nudelman, H]]
+[[Category: Kolusheva S]]
-[[Category: Tercedor, C V]]
+[[Category: Levi H]]
-[[Category: Widdrat, M]]
+[[Category: Nelkenbaum O]]
-[[Category: Zarivach, R]]
+[[Category: Nudelman H]]
-[[Category: Biomineralization]]
+[[Category: Tercedor CV]]
-[[Category: Magnetite]]
+[[Category: Widdrat M]]
-[[Category: Magnetite binding protein]]
+[[Category: Zarivach R]]
-[[Category: Magnetotactic bacteria]]
-[[Category: Mamc]]
-[[Category: Protein-mineral interaction]]

5i69

From Proteopedia

Current revision

MBP-MamC magnetite-interaction component mutant-D70A

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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