1hl3

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[[Image:1hl3.jpg|left|200px]]
 
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{{Structure
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==CtBP/BARS in ternary complex with NAD(H) and PIDLSKK peptide==
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|PDB= 1hl3 |SIZE=350|CAPTION= <scene name='initialview01'>1hl3</scene>, resolution 3.1&Aring;
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<StructureSection load='1hl3' size='340' side='right'caption='[[1hl3]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:Nad+Binding+Site+For+Chain+A'>AC1</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
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<table><tr><td colspan='2'>[[1hl3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HL3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HL3 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hl3 OCA], [https://pdbe.org/1hl3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hl3 RCSB], [https://www.ebi.ac.uk/pdbsum/1hl3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hl3 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hl3 OCA], [http://www.ebi.ac.uk/pdbsum/1hl3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hl3 RCSB]</span>
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== Function ==
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[https://www.uniprot.org/uniprot/CTBP1_RAT CTBP1_RAT] Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity. Functions in brown adipose tissue (BAT) differentiation.<ref>PMID:10364211</ref> <ref>PMID:7624370</ref> <ref>PMID:12805226</ref>
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== Evolutionary Conservation ==
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'''CTBP/BARS IN TERNARY COMPLEX WITH NAD(H) AND PIDLSKK PEPTIDE'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/1hl3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hl3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
C-terminal-binding protein/brefeldin A-ADP ribosylated substrate (CtBP/BARS) plays key roles in development and oncogenesis as a transcription co-repressor, and in intracellular traffic as a promoter of Golgi membrane fission. Co-repressor activity is regulated by NAD(H) binding to CtBP/BARS, while membrane fission is associated with its acyl-CoA-dependent acyltransferase activity. Here, we report the crystal structures of rat CtBP/BARS in a binary complex with NAD(H), and in a ternary complex with a PIDLSKK peptide mimicking the consensus motif (PXDLS) recognized in CtBP/BARS cellular partners. The structural data show CtBP/BARS in a NAD(H)-bound dimeric form; the peptide binding maps the recognition site for DNA-binding proteins and histone deacetylases to an N-terminal region of the protein. The crystal structure together with the site-directed mutagenesis data and binding experiments suggest a rationale for the molecular mechanisms underlying the two fundamental co-existing, but diverse, activities supported by CtBP/BARS in the nucleus and in Golgi membranes.
C-terminal-binding protein/brefeldin A-ADP ribosylated substrate (CtBP/BARS) plays key roles in development and oncogenesis as a transcription co-repressor, and in intracellular traffic as a promoter of Golgi membrane fission. Co-repressor activity is regulated by NAD(H) binding to CtBP/BARS, while membrane fission is associated with its acyl-CoA-dependent acyltransferase activity. Here, we report the crystal structures of rat CtBP/BARS in a binary complex with NAD(H), and in a ternary complex with a PIDLSKK peptide mimicking the consensus motif (PXDLS) recognized in CtBP/BARS cellular partners. The structural data show CtBP/BARS in a NAD(H)-bound dimeric form; the peptide binding maps the recognition site for DNA-binding proteins and histone deacetylases to an N-terminal region of the protein. The crystal structure together with the site-directed mutagenesis data and binding experiments suggest a rationale for the molecular mechanisms underlying the two fundamental co-existing, but diverse, activities supported by CtBP/BARS in the nucleus and in Golgi membranes.
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==About this Structure==
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CtBP/BARS: a dual-function protein involved in transcription co-repression and Golgi membrane fission.,Nardini M, Spano S, Cericola C, Pesce A, Massaro A, Millo E, Luini A, Corda D, Bolognesi M EMBO J. 2003 Jun 16;22(12):3122-30. PMID:12805226<ref>PMID:12805226</ref>
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1HL3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HL3 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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CtBP/BARS: a dual-function protein involved in transcription co-repression and Golgi membrane fission., Nardini M, Spano S, Cericola C, Pesce A, Massaro A, Millo E, Luini A, Corda D, Bolognesi M, EMBO J. 2003 Jun 16;22(12):3122-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12805226 12805226]
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</div>
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<div class="pdbe-citations 1hl3" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Synthetic construct]]
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[[Category: Bolognesi, M.]]
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[[Category: Bolognesi M]]
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[[Category: Cericola, C.]]
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[[Category: Cericola C]]
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[[Category: Corda, D.]]
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[[Category: Corda D]]
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[[Category: Luini, A.]]
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[[Category: Luini A]]
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[[Category: Massaro, A.]]
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[[Category: Massaro A]]
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[[Category: Millo, E.]]
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[[Category: Millo E]]
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[[Category: Nardini, M.]]
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[[Category: Nardini M]]
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[[Category: Pesce, A.]]
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[[Category: Pesce A]]
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[[Category: Spano , S.]]
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[[Category: Spano S]]
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[[Category: acyl-coa]]
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[[Category: acyltransferase]]
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[[Category: brefeldin some]]
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[[Category: golgi membrane]]
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[[Category: nad]]
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[[Category: transcription co-repression]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:07:02 2008''
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Current revision

CtBP/BARS in ternary complex with NAD(H) and PIDLSKK peptide

PDB ID 1hl3

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