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| | ==Structure of the soluble domain of EccB1 from the Mycobacterium smegmatis ESX-1 secretion system.== | | ==Structure of the soluble domain of EccB1 from the Mycobacterium smegmatis ESX-1 secretion system.== |
| - | <StructureSection load='5cyu' size='340' side='right' caption='[[5cyu]], [[Resolution|resolution]] 3.07Å' scene=''> | + | <StructureSection load='5cyu' size='340' side='right'caption='[[5cyu]], [[Resolution|resolution]] 3.07Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5cyu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CYU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CYU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5cyu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CYU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CYU FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cyu OCA], [http://pdbe.org/5cyu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cyu RCSB], [http://www.ebi.ac.uk/pdbsum/5cyu PDBsum]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.07Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cyu OCA], [https://pdbe.org/5cyu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cyu RCSB], [https://www.ebi.ac.uk/pdbsum/5cyu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cyu ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/ECCB1_MYCS2 ECCB1_MYCS2] An ATPase (shown for residues 72-479) (PubMed:26396239). Part of the ESX-1 / type VII specialized secretion system (T7SS), which exports several proteins including EsxA and EsxB (PubMed:15687187). Plays a role in DNA conjugation, in both donor and recipient strains (PubMed:15314236).<ref>PMID:15314236</ref> <ref>PMID:15687187</ref> <ref>PMID:26396239</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arbing, M A]] | + | [[Category: Large Structures]] |
| - | [[Category: Chan, S]] | + | [[Category: Mycolicibacterium smegmatis MC2 155]] |
| - | [[Category: Eisenberg, D S]] | + | [[Category: Arbing MA]] |
| - | [[Category: Kahng, S]] | + | [[Category: Chan S]] |
| - | [[Category: Kim, J]] | + | [[Category: Eisenberg DS]] |
| - | [[Category: Structural genomic]] | + | [[Category: Kahng S]] |
| - | [[Category: Membrane protein]] | + | [[Category: Kim J]] |
| - | [[Category: Mycobacteria]]
| + | |
| - | [[Category: Protein secretion]]
| + | |
| - | [[Category: PSI, Protein structure initiative]]
| + | |
| - | [[Category: Tbsgc]]
| + | |
| - | [[Category: Virulence]]
| + | |
| Structural highlights
Function
ECCB1_MYCS2 An ATPase (shown for residues 72-479) (PubMed:26396239). Part of the ESX-1 / type VII specialized secretion system (T7SS), which exports several proteins including EsxA and EsxB (PubMed:15687187). Plays a role in DNA conjugation, in both donor and recipient strains (PubMed:15314236).[1] [2] [3]
Publication Abstract from PubMed
BACKGROUND: The ESX-1 type VII secretion system is an important determinant of virulence in pathogenic mycobacteria, including Mycobacterium tuberculosis. This complicated molecular machine secretes folded proteins through the mycobacterial cell envelope to subvert the host immune response. Despite its important role in disease very little is known about the molecular architecture of the ESX-1 secretion system. RESULTS: This study characterizes the structures of the soluble domains of two conserved core ESX-1 components - EccB1 and EccD1. The periplasmic domain of EccB1 consists of 4 repeat domains and a central domain, which together form a quasi 2-fold symmetrical structure. The repeat domains of EccB1 are structurally similar to a known peptidoglycan binding protein suggesting a role in anchoring the ESX-1 system within the periplasmic space. The cytoplasmic domain of EccD1has a ubiquitin-like fold and forms a dimer with a negatively charged groove. CONCLUSIONS: These structures represent a major step towards resolving the molecular architecture of the entire ESX-1 assembly and may contribute to ESX-1 targeted tuberculosis intervention strategies.
Structures of EccB1 and EccD1 from the core complex of the mycobacterial ESX-1 type VII secretion system.,Wagner JM, Chan S, Evans TJ, Kahng S, Kim J, Arbing MA, Eisenberg D, Korotkov KV BMC Struct Biol. 2016 Feb 27;16(1):5. doi: 10.1186/s12900-016-0056-6. PMID:26922638[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Flint JL, Kowalski JC, Karnati PK, Derbyshire KM. The RD1 virulence locus of Mycobacterium tuberculosis regulates DNA transfer in Mycobacterium smegmatis. Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12598-603. PMID:15314236 doi:10.1073/pnas.0404892101
- ↑ Converse SE, Cox JS. A protein secretion pathway critical for Mycobacterium tuberculosis virulence is conserved and functional in Mycobacterium smegmatis. J Bacteriol. 2005 Feb;187(4):1238-45. PMID:15687187 doi:10.1128/JB.187.4.1238-1245.2005
- ↑ Zhang XL, Li DF, Fleming J, Wang LW, Zhou Y, Wang DC, Zhang XE, Bi LJ. Core component EccB1 of the Mycobacterium tuberculosis type VII secretion system is a periplasmic ATPase. FASEB J. 2015 Dec;29(12):4804-14. doi: 10.1096/fj.15-270843. Epub 2015 Sep 22. PMID:26396239 doi:http://dx.doi.org/10.1096/fj.15-270843
- ↑ Wagner JM, Chan S, Evans TJ, Kahng S, Kim J, Arbing MA, Eisenberg D, Korotkov KV. Structures of EccB1 and EccD1 from the core complex of the mycobacterial ESX-1 type VII secretion system. BMC Struct Biol. 2016 Feb 27;16(1):5. doi: 10.1186/s12900-016-0056-6. PMID:26922638 doi:http://dx.doi.org/10.1186/s12900-016-0056-6
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