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| - | [[Image:1hmh.jpg|left|200px]] | |
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| - | {{Structure
| + | ==THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME== |
| - | |PDB= 1hmh |SIZE=350|CAPTION= <scene name='initialview01'>1hmh</scene>, resolution 2.600Å
| + | <StructureSection load='1hmh' size='340' side='right'caption='[[1hmh]], [[Resolution|resolution]] 2.60Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| + | <table><tr><td colspan='2'>[[1hmh]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HMH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HMH FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hmh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hmh OCA], [https://pdbe.org/1hmh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hmh RCSB], [https://www.ebi.ac.uk/pdbsum/1hmh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hmh ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hmh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hmh OCA], [http://www.ebi.ac.uk/pdbsum/1hmh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hmh RCSB]</span>
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| - | }}
| + | |
| | | | |
| - | '''THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME'''
| + | ==See Also== |
| - | | + | *[[Ribozyme 3D structures|Ribozyme 3D structures]] |
| - | | + | __TOC__ |
| - | ==Overview==
| + | </StructureSection> |
| - | The hammerhead ribozyme is a small catalytic RNA motif made up of three base-paired stems and a core of highly conserved, non-complementary nucleotides essential for catalysis. The X-ray crystallographic structure of a hammerhead RNA-DNA ribozyme-inhibitor complex at 2.6 A resolution reveals that the base-paired stems are A-form helices and that the core has two structural domains. The first domain is formed by the sequence 5'-CUGA following stem I and is a sharp turn identical to the uridine turn of transfer RNA, whereas the second is a non-Watson-Crick three-base-pair duplex with a divalent-ion binding site. The phosphodiester backbone of the DNA inhibitor strand is splayed out at the phosphate 5' to the cleavage site. The structure indicates that the ribozyme may destabilize a substrate strand in order to facilitate twisting of the substrate to allow cleavage of the scissile bond.
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Flaherty KM]] |
| - | ==About this Structure== | + | [[Category: McKay DB]] |
| - | 1HMH is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HMH OCA].
| + | [[Category: Pley HW]] |
| - | | + | |
| - | ==Reference==
| + | |
| - | Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7969422 7969422]
| + | |
| - | [[Category: Protein complex]] | + | |
| - | [[Category: Flaherty, K M.]] | + | |
| - | [[Category: McKay, D B.]] | + | |
| - | [[Category: Pley, H W.]] | + | |
| - | [[Category: dna-rna hammerhead ribozyme]]
| + | |
| - | [[Category: loop]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:07:39 2008''
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