1hq0

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[[Image:1hq0.jpg|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF E.COLI CYTOTOXIC NECROTIZING FACTOR TYPE 1==
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|PDB= 1hq0 |SIZE=350|CAPTION= <scene name='initialview01'>1hq0</scene>, resolution 1.83&Aring;
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<StructureSection load='1hq0' size='340' side='right'caption='[[1hq0]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
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<table><tr><td colspan='2'>[[1hq0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HQ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HQ0 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.83&#8491;</td></tr>
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|GENE= CNF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hq0 OCA], [https://pdbe.org/1hq0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hq0 RCSB], [https://www.ebi.ac.uk/pdbsum/1hq0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hq0 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1hzg|1HZG]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hq0 OCA], [http://www.ebi.ac.uk/pdbsum/1hq0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hq0 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q47106_ECOLX Q47106_ECOLX]
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== Evolutionary Conservation ==
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'''CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF E.COLI CYTOTOXIC NECROTIZING FACTOR TYPE 1'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hq/1hq0_consurf.spt"</scriptWhenChecked>
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Certain uropathogenic and neonatal meningitis-causing strains of Escherichia coli express a 114 kDa protein toxin called cytotoxic necrotizing factor 1 (CNF1). The toxin causes alteration of the host cell actin cytoskeleton and promotes bacterial invasion of blood-brain barrier endothelial cells. CNF1 belongs to a unique group of large cytotoxins that cause constitutive activation of Rho guanosine triphosphatases (GTPases), which are key regulators of the actin cytoskeleton. This group also includes E. coli cytotoxic necrotizing factor 2 (CNF2, 114 kDa) and dermonecrotic toxins (DNT, 159 kDa) of Bordetella spp. with related sequences occurring in Yersinia spp. Here we show that the catalytic region of CNF1 exhibits a novel protein fold as determined by its 1.83 A resolution crystal structure. The structure reveals that CNF1 has a Cys-His-main chain oxygen catalytic triad reminiscent of enzymes belonging to the catalytic triad superfamily. The position of the catalytic Cys residue at the base of a deep pocket restricts access to potential substrates and helps explain the high specificity of this and related toxins.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1HQ0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HQ0 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hq0 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Structure of the Rho-activating domain of Escherichia coli cytotoxic necrotizing factor 1., Buetow L, Flatau G, Chiu K, Boquet P, Ghosh P, Nat Struct Biol. 2001 Jul;8(7):584-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11427886 11427886]
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Boquet, P.]]
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[[Category: Boquet P]]
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[[Category: Buetow, L.]]
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[[Category: Buetow L]]
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[[Category: Chiu, K.]]
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[[Category: Chiu K]]
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[[Category: Flatau, G.]]
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[[Category: Flatau G]]
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[[Category: Ghosh, P.]]
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[[Category: Ghosh P]]
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[[Category: beta sandwich]]
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[[Category: rho deamidase]]
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[[Category: rho transglutaminase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:08:52 2008''
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Current revision

CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF E.COLI CYTOTOXIC NECROTIZING FACTOR TYPE 1

PDB ID 1hq0

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