5hiw
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Sorangium cellulosum So Ce56 cytochrome P450 260B1== | |
| + | <StructureSection load='5hiw' size='340' side='right'caption='[[5hiw]], [[Resolution|resolution]] 1.85Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5hiw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sorangium_cellulosum_So_ce56 Sorangium cellulosum So ce56]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HIW FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hiw OCA], [https://pdbe.org/5hiw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hiw RCSB], [https://www.ebi.ac.uk/pdbsum/5hiw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hiw ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A9FFA1_SORC5 A9FFA1_SORC5] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Myxobacterial CYP260B1 from Sorangium cellulosum was heterologously expressed in Escherichia coli and purified. The in vitro conversion of a small focused substrate library comprised of Delta4 C21-steroids and steroidal drugs using surrogate bovine redox partners shows that CYP260B1 is a novel steroid hydroxylase. CYP260B1 performs the regio- and stereoselective hydroxylation of the glucocorticoid cortodoxone (RSS) to produce 6beta-OH-RSS. The substrate-free crystal structure of CYP260B1 (PDB 5HIW) was resolved. Docking of the tested ligands into the crystal structure suggested that the C17 hydroxy moiety and the presence of either a keto or a hydroxy group at C11 determine the selectivity of hydroxylation. | ||
| - | + | Structure-function analysis for the hydroxylation of Delta4 C21-steroids by the myxobacterial CYP260B1.,Salamanca-Pinzon SG, Khatri Y, Carius Y, Keller L, Muller R, Lancaster CR, Bernhardt R FEBS Lett. 2016 Jun;590(12):1838-51. doi: 10.1002/1873-3468.12217. Epub 2016 Jun , 3. PMID:27177597<ref>PMID:27177597</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5hiw" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]] |
| - | [[Category: Salamanca-Pinzon | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Sorangium cellulosum So ce56]] | ||
| + | [[Category: Bernhardt R]] | ||
| + | [[Category: Carius Y]] | ||
| + | [[Category: Khatri Y]] | ||
| + | [[Category: Lancaster CRD]] | ||
| + | [[Category: Salamanca-Pinzon SG]] | ||
Current revision
Sorangium cellulosum So Ce56 cytochrome P450 260B1
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