5hsp

From Proteopedia

(Difference between revisions)

Current revision

MamM CTD M250L

Structural highlights

5hsp is a 2 chain structure with sequence from Magnetospirillum gryphiswaldense. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.79Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MAMM_MAGGM Essential for magnetosome formation; required for stable accumulation of MamB (PubMed:22007638). May nucleate iron crystal formation (Probable). Probably binds and transports iron. Binds divalent cations, possibly up to 3 Zn(2+) per dimer in vitro, probably iron in vivo (Probable) (PubMed:30811856). One of 7 genes (mamLQBIEMO) able to induce magnetosome membrane biogenesis; coexpression of mamLQRBIEMO in a deletion of the 17 gene mamAB operon restores magnetosome vesicle formation but not magnetite biosynthesis (PubMed:27286560).^[1] ^[2] ^[3] ^[4] ^[5] ^[6]

Publication Abstract from PubMed

Cation diffusion facilitators (CDF) are highly conserved, metal ion efflux transporters that maintain divalent transition metal cation homeostasis. Most CDF proteins contain two domains, the cation transporting transmembrane domain and the regulatory cytoplasmic C-terminal domain (CTD). MamM is a magnetosome-associated CDF protein essential for the biomineralization of magnetic iron-oxide particles in magnetotactic bacteria. To investigate the structure-function relationship of CDF cytoplasmic domains, we characterized a MamM M250P mutation that is synonymous with the disease-related mutation L349P of the human CDF protein ZnT-10. Our results show that the M250P exchange in MamM causes severe structural changes in its CTD resulting in abnormal reduced function. Our in vivo, in vitro and in silico studies indicate that the CTD fold is critical for CDF proteins' proper function and support the previously suggested role of the CDF cytoplasmic domain as a CDF regulatory element. Based on our results, we also suggest a mechanism for the effects of the ZnT-10 L349P mutation in human.

Disease-Homologous Mutation in the Cation Diffusion Facilitator Protein MamM Causes Single-Domain Structural Loss and Signifies Its Importance.,Barber-Zucker S, Uebe R, Davidov G, Navon Y, Sherf D, Chill JH, Kass I, Bitton R, Schuler D, Zarivach R Sci Rep. 2016 Aug 23;6:31933. doi: 10.1038/srep31933. PMID:27550551^[7]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Uebe R, Junge K, Henn V, Poxleitner G, Katzmann E, Plitzko JM, Zarivach R, Kasama T, Wanner G, Pósfai M, Böttger L, Matzanke B, Schüler D. The cation diffusion facilitator proteins MamB and MamM of Magnetospirillum gryphiswaldense have distinct and complex functions, and are involved in magnetite biomineralization and magnetosome membrane assembly. Mol Microbiol. 2011 Nov;82(4):818-35. PMID:22007638 doi:10.1111/j.1365-2958.2011.07863.x
↑ Raschdorf O, Forstner Y, Kolinko I, Uebe R, Plitzko JM, Schüler D. Genetic and Ultrastructural Analysis Reveals the Key Players and Initial Steps of Bacterial Magnetosome Membrane Biogenesis. PLoS Genet. 2016 Jun 10;12(6):e1006101. PMID:27286560 doi:10.1371/journal.pgen.1006101
↑ Barber-Zucker S, Hall J, Mangapuram SV, Kass I, Kolusheva S, MacMillan F, Zarivach R, Henn A. Metal binding to the dynamic cytoplasmic domain of the cation diffusion facilitator (CDF) protein MamM induces a 'locked-in' configuration. FEBS J. 2019 Feb 27. doi: 10.1111/febs.14795. PMID:30811856 doi:http://dx.doi.org/10.1111/febs.14795
↑ Uebe R, Junge K, Henn V, Poxleitner G, Katzmann E, Plitzko JM, Zarivach R, Kasama T, Wanner G, Pósfai M, Böttger L, Matzanke B, Schüler D. The cation diffusion facilitator proteins MamB and MamM of Magnetospirillum gryphiswaldense have distinct and complex functions, and are involved in magnetite biomineralization and magnetosome membrane assembly. Mol Microbiol. 2011 Nov;82(4):818-35. PMID:22007638 doi:10.1111/j.1365-2958.2011.07863.x
↑ Zeytuni N, Uebe R, Maes M, Davidov G, Baram M, Raschdorf O, Nadav-Tsubery M, Kolusheva S, Bitton R, Goobes G, Friedler A, Miller Y, Schuler D, Zarivach R. Cation diffusion facilitators transport initiation and regulation is mediated by cation induced conformational changes of the cytoplasmic domain. PLoS One. 2014 Mar 21;9(3):e92141. doi: 10.1371/journal.pone.0092141. eCollection, 2014. PMID:24658343 doi:http://dx.doi.org/10.1371/journal.pone.0092141
↑ Zeytuni N, Uebe R, Maes M, Davidov G, Baram M, Raschdorf O, Friedler A, Miller Y, Schuler D, Zarivach R. Bacterial Magnetosome Biomineralization - A Novel Platform to Study Molecular Mechanisms of Human CDF-Related Type-II Diabetes. PLoS One. 2014 May 12;9(5):e97154. doi: 10.1371/journal.pone.0097154. eCollection, 2014. PMID:24819161 doi:http://dx.doi.org/10.1371/journal.pone.0097154
↑ Barber-Zucker S, Uebe R, Davidov G, Navon Y, Sherf D, Chill JH, Kass I, Bitton R, Schuler D, Zarivach R. Disease-Homologous Mutation in the Cation Diffusion Facilitator Protein MamM Causes Single-Domain Structural Loss and Signifies Its Importance. Sci Rep. 2016 Aug 23;6:31933. doi: 10.1038/srep31933. PMID:27550551 doi:http://dx.doi.org/10.1038/srep31933

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5hsp"

Categories: Large Structures | Magnetospirillum gryphiswaldense | Barber-Zucker S | Zarivach R

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5hsp is ON HOLD
+==MamM CTD M250L==
+<StructureSection load='5hsp' size='340' side='right'caption='[[5hsp]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5hsp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Magnetospirillum_gryphiswaldense Magnetospirillum gryphiswaldense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HSP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HSP FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.79&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hsp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hsp OCA], [https://pdbe.org/5hsp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hsp RCSB], [https://www.ebi.ac.uk/pdbsum/5hsp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hsp ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MAMM_MAGGM MAMM_MAGGM] Essential for magnetosome formation; required for stable accumulation of MamB (PubMed:22007638). May nucleate iron crystal formation (Probable). Probably binds and transports iron. Binds divalent cations, possibly up to 3 Zn(2+) per dimer in vitro, probably iron in vivo (Probable) (PubMed:30811856). One of 7 genes (mamLQBIEMO) able to induce magnetosome membrane biogenesis; coexpression of mamLQRBIEMO in a deletion of the 17 gene mamAB operon restores magnetosome vesicle formation but not magnetite biosynthesis (PubMed:27286560).<ref>PMID:22007638</ref> <ref>PMID:27286560</ref> <ref>PMID:30811856</ref> <ref>PMID:22007638</ref> <ref>PMID:24658343</ref> <ref>PMID:24819161</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Cation diffusion facilitators (CDF) are highly conserved, metal ion efflux transporters that maintain divalent transition metal cation homeostasis. Most CDF proteins contain two domains, the cation transporting transmembrane domain and the regulatory cytoplasmic C-terminal domain (CTD). MamM is a magnetosome-associated CDF protein essential for the biomineralization of magnetic iron-oxide particles in magnetotactic bacteria. To investigate the structure-function relationship of CDF cytoplasmic domains, we characterized a MamM M250P mutation that is synonymous with the disease-related mutation L349P of the human CDF protein ZnT-10. Our results show that the M250P exchange in MamM causes severe structural changes in its CTD resulting in abnormal reduced function. Our in vivo, in vitro and in silico studies indicate that the CTD fold is critical for CDF proteins' proper function and support the previously suggested role of the CDF cytoplasmic domain as a CDF regulatory element. Based on our results, we also suggest a mechanism for the effects of the ZnT-10 L349P mutation in human.
-Authors: Barber-Zucker, S., Zarivach, R.
+Disease-Homologous Mutation in the Cation Diffusion Facilitator Protein MamM Causes Single-Domain Structural Loss and Signifies Its Importance.,Barber-Zucker S, Uebe R, Davidov G, Navon Y, Sherf D, Chill JH, Kass I, Bitton R, Schuler D, Zarivach R Sci Rep. 2016 Aug 23;6:31933. doi: 10.1038/srep31933. PMID:27550551<ref>PMID:27550551</ref>
-Description: MamM CTD M250L
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Zarivach, R]]
+<div class="pdbe-citations 5hsp" style="background-color:#fffaf0;"></div>
-[[Category: Barber-Zucker, S]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Magnetospirillum gryphiswaldense]]
+[[Category: Barber-Zucker S]]
+[[Category: Zarivach R]]

5hsp

From Proteopedia

Current revision

MamM CTD M250L

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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