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1hv6

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[[Image:1hv6.gif|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III COMPLEXED WITH TRISACCHARIDE PRODUCT.==
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|PDB= 1hv6 |SIZE=350|CAPTION= <scene name='initialview01'>1hv6</scene>, resolution 2.00&Aring;
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<StructureSection load='1hv6' size='340' side='right'caption='[[1hv6]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=GCU:D-GLUCURONIC+ACID'>GCU</scene>, <scene name='pdbligand=MAV:D-MANNURONIC+ACID'>MAV</scene>, <scene name='pdbligand=MAW:4-DEOXY-D-MANNURONIC+ACID'>MAW</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1hv6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingomonas_sp. Sphingomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HV6 FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(beta-D-mannuronate)_lyase Poly(beta-D-mannuronate) lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.3 4.2.2.3] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GCU:D-GLUCURONIC+ACID'>GCU</scene>, <scene name='pdbligand=MAV:ALPHA-D-MANNOPYRANURONIC+ACID'>MAV</scene>, <scene name='pdbligand=MAW:4-DEOXY-ALPHA-L-ERYTHRO-HEX-4-ENOPYRANURONIC+ACID'>MAW</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hv6 OCA], [https://pdbe.org/1hv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hv6 RCSB], [https://www.ebi.ac.uk/pdbsum/1hv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hv6 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1qaz|1QAZ]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hv6 OCA], [http://www.ebi.ac.uk/pdbsum/1hv6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hv6 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q9KWU1_SPHSX Q9KWU1_SPHSX]
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== Evolutionary Conservation ==
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'''CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III COMPLEXED WITH TRISACCHARIDE PRODUCT.'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hv/1hv6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hv6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The structure of A1-III from a Sphingomonas species A1 complexed with a trisaccharide product (4-deoxy-l-erythro-hex-4-enepyranosyluronate-mannuronate-mannuronic acid) was determined by X-ray crystallography at 2.0 A with an R-factor of 0.16. The final model of the complex form comprising 351 amino acid residues, 245 water molecules, one sulfate ion and one trisaccharide product exhibited a C(alpha) r.m.s.d. value of 0.154 A with the reported apo form of the enzyme. The trisaccharide was bound in the active cleft at subsites -3 approximately -1 from the non-reducing end by forming several hydrogen bonds and van der Waals interactions with protein atoms. The catalytic residue was estimated to be Tyr246, which existed between subsites -1 and +1 based on a mannuronic acid model oriented at subsite +1.
The structure of A1-III from a Sphingomonas species A1 complexed with a trisaccharide product (4-deoxy-l-erythro-hex-4-enepyranosyluronate-mannuronate-mannuronic acid) was determined by X-ray crystallography at 2.0 A with an R-factor of 0.16. The final model of the complex form comprising 351 amino acid residues, 245 water molecules, one sulfate ion and one trisaccharide product exhibited a C(alpha) r.m.s.d. value of 0.154 A with the reported apo form of the enzyme. The trisaccharide was bound in the active cleft at subsites -3 approximately -1 from the non-reducing end by forming several hydrogen bonds and van der Waals interactions with protein atoms. The catalytic residue was estimated to be Tyr246, which existed between subsites -1 and +1 based on a mannuronic acid model oriented at subsite +1.
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==About this Structure==
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Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 A resolution.,Yoon HJ, Hashimoto W, Miyake O, Murata K, Mikami B J Mol Biol. 2001 Mar 16;307(1):9-16. PMID:11243798<ref>PMID:11243798</ref>
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1HV6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sphingomonas_species Sphingomonas species]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HV6 OCA].
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==Reference==
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Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 A resolution., Yoon HJ, Hashimoto W, Miyake O, Murata K, Mikami B, J Mol Biol. 2001 Mar 16;307(1):9-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11243798 11243798]
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[[Category: Poly(beta-D-mannuronate) lyase]]
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[[Category: Single protein]]
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[[Category: Sphingomonas species]]
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[[Category: Hashimoto, W.]]
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[[Category: Mikami, B.]]
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[[Category: Miyake, O.]]
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[[Category: Murata, K.]]
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[[Category: Yoon, H J.]]
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[[Category: alginate lyase]]
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[[Category: trisaccharide complex,alpha barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:10:50 2008''
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1hv6" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Sphingomonas sp]]
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[[Category: Hashimoto W]]
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[[Category: Mikami B]]
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[[Category: Miyake O]]
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[[Category: Murata K]]
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[[Category: Yoon H-J]]

Current revision

CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III COMPLEXED WITH TRISACCHARIDE PRODUCT.

PDB ID 1hv6

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