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5iy6

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'''Unreleased structure'''
 
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The entry 5iy6 is ON HOLD
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==Human holo-PIC in the closed state==
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<SX load='5iy6' size='340' side='right' viewer='molstar' caption='[[5iy6]], [[Resolution|resolution]] 7.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5iy6]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IY6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IY6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5iy6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iy6 OCA], [https://pdbe.org/5iy6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5iy6 RCSB], [https://www.ebi.ac.uk/pdbsum/5iy6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5iy6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/T2FB_HUMAN T2FB_HUMAN] TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.<ref>PMID:2477704</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In eukaryotic transcription initiation, a large multi-subunit pre-initiation complex (PIC) that assembles at the core promoter is required for the opening of the duplex DNA and identification of the start site for transcription by RNA polymerase II. Here we use cryo-electron microscropy (cryo-EM) to determine near-atomic resolution structures of the human PIC in a closed state (engaged with duplex DNA), an open state (engaged with a transcription bubble), and an initially transcribing complex (containing six base pairs of DNA-RNA hybrid). Our studies provide structures for previously uncharacterized components of the PIC, such as TFIIE and TFIIH, and segments of TFIIA, TFIIB and TFIIF. Comparison of the different structures reveals the sequential conformational changes that accompany the transition from each state to the next throughout the transcription initiation process. This analysis illustrates the key role of TFIIB in transcription bubble stabilization and provides strong structural support for a translocase activity of XPB.
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Authors: He, Y., Yan, C., Fang, J., Inouye, C., Tjian, R., Ivanov, I., Nogales, E.
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Near-atomic resolution visualization of human transcription promoter opening.,He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E Nature. 2016 May 11;533(7603):359-65. doi: 10.1038/nature17970. PMID:27193682<ref>PMID:27193682</ref>
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Description: Human holo-PIC in the closed state
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Nogales, E]]
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<div class="pdbe-citations 5iy6" style="background-color:#fffaf0;"></div>
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[[Category: Tjian, R]]
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[[Category: He, Y]]
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==See Also==
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[[Category: Ivanov, I]]
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*[[Helicase 3D structures|Helicase 3D structures]]
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[[Category: Inouye, C]]
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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[[Category: Yan, C]]
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*[[TATA-binding protein 3D structures|TATA-binding protein 3D structures]]
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[[Category: Fang, J]]
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*[[Transcription initiation factors 3D structures|Transcription initiation factors 3D structures]]
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== References ==
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<references/>
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__TOC__
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</SX>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Fang J]]
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[[Category: He Y]]
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[[Category: Inouye C]]
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[[Category: Ivanov I]]
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[[Category: Nogales E]]
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[[Category: Tjian R]]
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[[Category: Yan C]]

Current revision

Human holo-PIC in the closed state

5iy6, resolution 7.20Å

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