1hwy

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[[Image:1hwy.gif|left|200px]]
 
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{{Structure
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==BOVINE GLUTAMATE DEHYDROGENASE COMPLEXED WITH NAD AND 2-OXOGLUTARATE==
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|PDB= 1hwy |SIZE=350|CAPTION= <scene name='initialview01'>1hwy</scene>, resolution 3.2&Aring;
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<StructureSection load='1hwy' size='340' side='right'caption='[[1hwy]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=AKG:2-OXYGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
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<table><tr><td colspan='2'>[[1hwy]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HWY FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate_dehydrogenase_(NAD(P)(+)) Glutamate dehydrogenase (NAD(P)(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.1.3 1.4.1.3] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwy OCA], [https://pdbe.org/1hwy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hwy RCSB], [https://www.ebi.ac.uk/pdbsum/1hwy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hwy ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1hwx|1HWX]], [[1hwz|1HWZ]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwy OCA], [http://www.ebi.ac.uk/pdbsum/1hwy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hwy RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/DHE3_BOVIN DHE3_BOVIN] May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).<ref>PMID:14659072</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hw/1hwy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hwy ConSurf].
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<div style="clear:both"></div>
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'''BOVINE GLUTAMATE DEHYDROGENASE COMPLEXED WITH NAD AND 2-OXOGLUTARATE'''
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==See Also==
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*[[Glutamate dehydrogenase 3D structures|Glutamate dehydrogenase 3D structures]]
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== References ==
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==Overview==
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<references/>
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Glutamate dehydrogenase is found in all organisms and catalyses the oxidative deamination of l-glutamate to 2-oxoglutarate. However, only animal GDH utilizes both NAD(H) or NADP(H) with comparable efficacy and exhibits a complex pattern of allosteric inhibition by a wide variety of small molecules. The major allosteric inhibitors are GTP and NADH and the two main allosteric activators are ADP and NAD(+). The structures presented here have refined and modified the previous structural model of allosteric regulation inferred from the original boGDH.NADH.GLU.GTP complex. The boGDH.NAD(+).alpha-KG complex structure clearly demonstrates that the second coenzyme-binding site lies directly under the "pivot helix" of the NAD(+) binding domain. In this complex, phosphates are observed to occupy the inhibitory GTP site and may be responsible for the previously observed structural stabilization by polyanions. The boGDH.NADPH.GLU.GTP complex shows the location of the additional phosphate on the active site coenzyme molecule and the GTP molecule bound to the GTP inhibitory site. As expected, since NADPH does not bind well to the second coenzyme site, no evidence of a bound molecule is observed at the second coenzyme site under the pivot helix. Therefore, these results suggest that the inhibitory GTP site is as previously identified. However, ADP, NAD(+), and NADH all bind under the pivot helix, but a second GTP molecule does not. Kinetic analysis of a hyperinsulinism/hyperammonemia mutant strongly suggests that ATP can inhibit the reaction by binding to the GTP site. Finally, the fact that NADH, NAD(+), and ADP all bind to the same site requires a re-analysis of the previous models for NADH inhibition.
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__TOC__
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</StructureSection>
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==About this Structure==
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1HWY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWY OCA].
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==Reference==
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Structures of bovine glutamate dehydrogenase complexes elucidate the mechanism of purine regulation., Smith TJ, Peterson PE, Schmidt T, Fang J, Stanley CA, J Mol Biol. 2001 Mar 23;307(2):707-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11254391 11254391]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Glutamate dehydrogenase (NAD(P)(+))]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Fang J]]
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[[Category: Fang, J.]]
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[[Category: Peterson PE]]
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[[Category: Peterson, P E.]]
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[[Category: Schmidt T]]
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[[Category: Schmidt, T.]]
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[[Category: Smith TJ]]
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[[Category: Smith, T J.]]
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[[Category: Stanley CA]]
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[[Category: Stanley, C A.]]
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[[Category: allostery]]
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[[Category: glutamate dehydrogenase]]
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[[Category: nad]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:11:29 2008''
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Current revision

BOVINE GLUTAMATE DEHYDROGENASE COMPLEXED WITH NAD AND 2-OXOGLUTARATE

PDB ID 1hwy

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