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5j8x

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'''Unreleased structure'''
 
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The entry 5j8x is ON HOLD until Paper Publication
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==CRYSTAL STRUCTURE OF E. COLI PBP5 WITH 2C==
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<StructureSection load='5j8x' size='340' side='right'caption='[[5j8x]], [[Resolution|resolution]] 2.53&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5j8x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J8X FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.53&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OK3:(R)-3-(4-(AMINOMETHYL)BENZAMIDO)-8-CARBOXY-2,2-DIHYDROXY-3,4-DIHYDRO-2H-BENZO[E][1,2]OXABORININ-2-UIDE'>OK3</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j8x OCA], [https://pdbe.org/5j8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j8x RCSB], [https://www.ebi.ac.uk/pdbsum/5j8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j8x ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DACA_ECOLI DACA_ECOLI] Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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beta-Lactamases enable resistance to almost all beta-lactam antibiotics. Pioneering work revealed that acyclic boronic acids can act as 'transition state analogue' inhibitors of nucleophilic serine enzymes, including serine-beta-lactamases. Here we report biochemical and biophysical analyses revealing that cyclic boronates potently inhibit both nucleophilic serine and zinc-dependent beta-lactamases by a mechanism involving mimicking of the common tetrahedral intermediate. Cyclic boronates also potently inhibit the non-essential penicillin-binding protein PBP 5 by the same mechanism of action. The results open the way for development of dual action inhibitors effective against both serine- and metallo-beta-lactamases, and which could also have antimicrobial activity through inhibition of PBPs.
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Authors: Brem, J., Cain, R., McDonough, M.A., Clifton, I.J., Fishwick, C.W.G., Schofield, C.J.
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Structural basis of metallo-beta-lactamase, serine-beta-lactamase and penicillin-binding protein inhibition by cyclic boronates.,Brem J, Cain R, Cahill S, McDonough MA, Clifton IJ, Jimenez-Castellanos JC, Avison MB, Spencer J, Fishwick CW, Schofield CJ Nat Commun. 2016 Aug 8;7:12406. doi: 10.1038/ncomms12406. PMID:27499424<ref>PMID:27499424</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Clifton, I.J]]
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<div class="pdbe-citations 5j8x" style="background-color:#fffaf0;"></div>
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[[Category: Mcdonough, M.A]]
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[[Category: Fishwick, C.W.G]]
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==See Also==
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[[Category: Cain, R]]
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*[[Carboxypeptidase 3D structures|Carboxypeptidase 3D structures]]
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[[Category: Schofield, C.J]]
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== References ==
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[[Category: Brem, J]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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[[Category: Brem J]]
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[[Category: Cain R]]
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[[Category: Clifton IJ]]
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[[Category: Fishwick CWG]]
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[[Category: McDonough MA]]
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[[Category: Schofield CJ]]

Current revision

CRYSTAL STRUCTURE OF E. COLI PBP5 WITH 2C

PDB ID 5j8x

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